Overview

  • Product nameAnti-Cyclin B1 antibodySee all Cyclin B1 primary antibodies ...
  • Description
    Rabbit polyclonal to Cyclin B1
  • SpecificityAntiserum will specifically react with a 62 kDa Cyclin B1 protein. No reaction was observed against other related cyclins.
  • Tested applicationsWB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein (Human).

Properties

Applications

Our Abpromise guarantee covers the use of ab2096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 48 kDa).
IP Use at an assay dependent dilution.
ELISA 1/500 - 1/2000.

Target

References for Anti-Cyclin B1 antibody (ab2096)

This product has been referenced in:
  • Kotecha M  et al. Inhibition of DNA binding of the NF-Y transcription factor by the pyrrolobenzodiazepine-polyamide conjugate GWL-78. Mol Cancer Ther 7:1319-28 (2008). WB ; Mouse . Read more (PubMed: 18483319) »
  • Morris C & Jalinot P Silencing of human Int-6 impairs mitosis progression and inhibits cyclin B-Cdk1 activation. Oncogene 24:1203-11 (2005). Read more (PubMed: 15558017) »

See all 2 Publications for this product

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Xenopus laevis Cell lysate - whole cell (embryos before MBT)
Loading amount 1 cells
Specification embryos before MBT
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Jolanta Kisielewska

Verified customer

Submitted Apr 16 2010

Antibodies ab2096 (anti-cyclin B1) and ab2094 (anti-cyclin E) have not been tested in immunofluorescence. Ab72 (monoclonal to cyclin B1, V152) can be used for histology.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"