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Synthetic peptide From C terminal (Human)
Our Abpromise guarantee covers the use of ab2949 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution.|
|Sandwich ELISA||Use a concentration of 0.2 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [7A9] to Cyclin B1 (ab49215). For sandwich ELISA, use this antibody as Detection at 0.2 µg/ml with Mouse monoclonal [7A9] to Cyclin B1 (ab49215) as Capture.|
|IHC-P||Use a concentration of 10 - 20 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IP||Use at 10 µg/mg of lysate.|
|WB||Use at an assay dependent dilution. Detects a band of approximately 60 kDa (predicted molecular weight: 48 kDa).|
ab2949 staining Cyclin B1 in mouse MEF cells by Immunocytochemistry/ immunoflurescence. Cells were fixed with paraformaldehyde and blocking with 2% serum was performed for 2 hours at 250C. Samples were incubated with primary antibody (1/100: in 1% BSA, 0.3% Triton X-100 in PBS) for 18 hours at 4°C. An Alexa Fluor®594-conjugated goat polyclonal to rabbit IgG was used diluted 1/1000 as secondary antibody. Red is cyclinB1 staining, blue is DAPI staining DNA for nuclei, green is vinculin staining.
ab2949 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"