Overview

  • Product nameAnti-Cyclin B1 antibodySee all Cyclin B1 primary antibodies ...
  • Description
    Rabbit polyclonal to Cyclin B1
  • Tested applicationsICC/IF, Sandwich ELISA, IHC-P, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide From C terminal (Human)

  • Positive control
    • Jurkat, Ls174T or HeLa cells. Breast carcinoma tissue section.

Properties

Applications

Our Abpromise guarantee covers the use of ab2949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
Sandwich ELISA Use a concentration of 0.2 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [7A9] to Cyclin B1 (ab49215). For sandwich ELISA, use this antibody as Detection at 0.2 µg/ml with Mouse monoclonal [7A9] to Cyclin B1 (ab49215) as Capture.
IHC-P Use a concentration of 10 - 20 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP Use at 10 µg/mg of lysate.
WB Use at an assay dependent dilution. Detects a band of approximately 60 kDa (predicted molecular weight: 48 kDa).

Target

Anti-Cyclin B1 antibody images

  • Standard Curve for Cyclin B1 (Analyte: Cyclin B1 protein (His tag) (ab40591)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [7A9] to Cyclin B1 (ab49215) at 0.2µg/ml and Detector Antibody Rabbit polyclonal to Cyclin B1 (ab2949) at 0.2µg/ml.
  • Breast carcinoma stained with ab2949
  • ab2949 staining Cyclin B1 in mouse MEF cells by Immunocytochemistry/ immunoflurescence. Cells were fixed with paraformaldehyde and blocking with 2% serum was performed for 2 hours at 250C. Samples were incubated with primary antibody (1/100: in 1% BSA, 0.3% Triton X-100 in PBS) for 18 hours at 4°C. An Alexa Fluor®594-conjugated goat polyclonal to rabbit IgG was used diluted 1/1000 as secondary antibody. Red is cyclinB1 staining, blue is DAPI staining DNA for nuclei, green is vinculin staining.

    See Abreview

Protocols

References for Anti-Cyclin B1 antibody (ab2949)

This product has been referenced in:
  • Hardy T  et al. Multisite phosphorylation of C-Nap1 releases it from Cep135 to trigger centrosome disjunction. J Cell Sci 127:2493-506 (2014). ICC/IF ; Human . Read more (PubMed: 24695856) »

See 1 Publication for this product

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Unfortunately the immunogen sequence is confidential. It is located within aa 370-433 of P14635 (Uniprot).

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Expiration date: 5th December 2012

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Application Western blot
Sample Rat Tissue lysate - whole (Liver)
Loading amount 50 µg
Specification Liver
Gel Running Conditions Reduced Denaturing (12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Sep 05 2011

Application Western blot
Sample Mouse Tissue lysate - whole (Liver)
Loading amount 50 µg
Specification Liver
Gel Running Conditions Reduced Denaturing (12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Aug 24 2011

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (MEF)
Specification MEF
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 28 2009

Thank you for your e-mail and forwarding more information about your Western blot assay. We are very sorry to hear that you are having problem with this antibody. We have searched our database and found that this is a popular selling product and yo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"