Overview

  • Product nameAnti-Cyclin B1 antibodySee all Cyclin B1 primary antibodies ...
  • Description
    Rabbit polyclonal to Cyclin B1
  • Tested applicationsIHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide derived from C-terminus of human Cyclin B1.

  • Positive control
    • Breast carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab48574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC method.

Target

Anti-Cyclin B1 antibody images

  • Ab48574 staining human Cyclin B1 in human breast carcinoma by immunohistochemistry using formalin fixed paraffin embedded tissue.
  • Ab48574 at 1/100 dilution staining Cyclin B1 in rat E19 embryonic brain sections by immunohistochemistry. Please see accompanying abreview for additional information.

    See Abreview

References for Anti-Cyclin B1 antibody (ab48574)

ab48574 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Cortical Brain Sections)
Specification Cortical Brain Sections
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization Yes - TBS with 1% triton
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 11 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (E19 Embryonic Brain Sections)
Specification E19 Embryonic Brain Sections
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization Yes - PBS + 1% trition
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 20 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"