Anti-Cyclin B1 antibody (ab52187)

Overview

• Product nameAnti-Cyclin B1 antibodySee all Cyclin B1 primary antibodies ...
• Description
  Rabbit polyclonal to Cyclin B1
• SpecificityDetects endogenous levels of total Cyclin B1 protein.
• Tested applicationsELISA, WB, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Synthetic peptide, corresponding to amino acids 111-150 of Human Cyclin B1.Synthetic non-phosphopeptide derived from human Cyclin B1 around the phosphorylation site of serine 126 (T-A-S-P-S).

• Positive controlExtracts from HeLa cells treated with serum.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Cell cycle
  • Cyclins
  • Cyclin B family

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Cyclins
  • Cyclin B Family

  • Cell Biology
  • Cell Cycle
  • Cyclins
  • Cyclin B Family

Applications

Target

• FunctionEssential for the control of the cell cycle at the G2/M (mitosis) transition.
• Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
• Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
• Post-translational
  modificationsUbiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
• Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.

Target information above from: UniProt accession P14635 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 891 Human
  • Entrez Gene: 268697 Mouse
  • Omim: 123836 Human
  • SwissProt: P14635 Human
  • SwissProt: P24860 Mouse
  • Unigene: 23960 Human
  • Unigene: 260114 Mouse
  • Unigene: 380027 Mouse

  • Unigene: 482545 Mouse

  see all

• Alternative names
  CCNB 1 antibodyCCNB antibodyCCNB1 antibody

  CCNB1_HUMAN antibodyG2 mitotic specific cyclin B1 antibodyG2/mitotic-specific cyclin-B1 antibody

  see all

Anti-Cyclin B1 antibody images

• 

  Western blot - Cyclin B1 antibody (ab52187)
  All lanes : Anti-Cyclin B1 antibody (ab52187) at 1/500 dilution
  
  Lane 1 : extracts from HeLa cells, treated with serum (10%, 15min)
  Lane 2 : extracts from HeLa cells, treated with serum (10%, 15min) with blocking peptide
  
  
  Predicted band size : 48 kDa
  Observed band size : 48 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Cyclin B1 antibody(ab52187)
  ab52187 (2µg/ml) staining Cyclin B1 in human placenta.
  Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 . Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.