Synthetic peptide corresponding to Human Cyclin B1. Immunogen range is aa 91-140.
Synthetic non-phosphopeptide derived from human Cyclin B1 around the phosphorylation site of serine 126 Database link: P14635
Extracts from HeLa cells treated with serum.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
ab52187 (2µg/ml) staining Cyclin B1 in human placenta. Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 . Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
References for Anti-Cyclin B1 antibody (ab52187)
This product has been referenced in:
Park JE et al. Direct quantification of polo-like kinase 1 activity in cells and tissues using a highly sensitive and specific ELISA assay. Proc Natl Acad Sci U S A106:1725-1730 (2009).
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