Overview
- Product nameAnti-Cyclin B1 antibodySee all Cyclin B1 primary antibodies ...
- DescriptionRabbit polyclonal to Cyclin B1
- Tested applicationsWB, ELISA more details
- Species reactivityReacts with: Human
- Positive controlNCI-H460 cell line lysates
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesThis antibody is purified through a protein A column, followed by peptide affinity purification.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab75952 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/50 - 1/100. Predicted molecular weight: 48 kDa. |
| ELISA | ELISA: 1/1000. |
Target
- FunctionEssential for the control of the cell cycle at the G2/M (mitosis) transition.
- Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
- Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
- Post-translational
modificationsUbiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases. - Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
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Database links
- Entrez Gene: 891 Human
- Omim: 123836 Human
- SwissProt: P14635 Human
- Unigene: 23960 Human
Target information above from: UniProt accession
P14635
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- CCNB 1 antibodyCCNB antibodyCCNB1 antibody
- CCNB1_HUMAN antibodyG2 mitotic specific cyclin B1 antibodyG2/mitotic-specific cyclin-B1 antibody
see all
Anti-Cyclin B1 antibody images
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Western blot - Cyclin B1 antibody (ab75952)Anti-Cyclin B1 antibody (ab75952) at 1/60 dilution + NCI-H460 cell line lysate at 35 µg
Predicted band size : 48 kDa
Observed band size : 48 kDa
References for Anti-Cyclin B1 antibody (ab75952)
ab75952 has not yet been referenced specifically in any publications.
