Overview
- Product nameAnti-Cyclin B1 antibodySee all Cyclin B1 primary antibodies ...
- DescriptionRabbit polyclonal to Cyclin B1
- Tested applicationsWB, ELISA more details
- Species reactivityReacts with: Mouse, Human
- Immunogen
Synthetic peptide corresponding to amino acid residues surrounding S126 of human Cyclin B1, conjugated to KLH.
- Positive controlA375 cell line lysate
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesThis antibody is purified through a protein A column, followed by peptide affinity purification.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab76017 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/50 - 1/100. Predicted molecular weight: 48 kDa. |
| ELISA | ELISA: 1/1000. |
Target
- FunctionEssential for the control of the cell cycle at the G2/M (mitosis) transition.
- Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
- Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
- Post-translational
modificationsUbiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases. - Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
-
Database links
- Entrez Gene: 891 Human
- Entrez Gene: 268697 Mouse
- Omim: 123836 Human
- SwissProt: P14635 Human
- SwissProt: P24860 Mouse
- Unigene: 23960 Human
- Unigene: 260114 Mouse
- Unigene: 380027 Mouse
- Unigene: 482545 Mouse
see all
Target information above from: UniProt accession
P14635
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
-
Alternative names
- CCNB 1 antibodyCCNB antibodyCCNB1 antibody
- CCNB1_HUMAN antibodyG2 mitotic specific cyclin B1 antibodyG2/mitotic-specific cyclin-B1 antibody
see all
Anti-Cyclin B1 antibody images
-
Western blot - Cyclin B1 antibody (ab76017)Anti-Cyclin B1 antibody (ab76017) at 1/50 dilution + A375 cell line lysates at 35 µg
Predicted band size : 48 kDa
Observed band size : 48 kDa
References for Anti-Cyclin B1 antibody (ab76017)
ab76017 has not yet been referenced specifically in any publications.
