Overview

  • Product nameAnti-Cyclin B1 antibodySee all Cyclin B1 primary antibodies ...
  • Description
    Rabbit polyclonal to Cyclin B1
  • SpecificityReacts with cyclin B1
  • Tested applicationsIHC-P, IHC-Fr, IP, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    TLPQLNSALVQDLAKAVAKV

    , corresponding to C terminal amino acids 414-433 of Human Cyclin B1.

  • Positive control
    • HeLa and K-562 IHC-P: FFPE human cervix tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab7957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 49 kDa).Can be blocked with Cyclin B1 peptide (ab8012).

Target

  • FunctionEssential for the control of the cell cycle at the G2/M (mitosis) transition.
  • Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
  • Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
  • Post-translational
    modifications
    Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCNB 1 antibody
    • CCNB antibody
    • CCNB1 antibody
    • CCNB1_HUMAN antibody
    • G2 mitotic specific cyclin B1 antibody
    • G2/mitotic-specific cyclin-B1 antibody
    see all

Anti-Cyclin B1 antibody images

  • IHC image of Cyclin B1 staining in human cervix formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7957, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.



  • Predicted band size : 49 kDa
    ab7957 staining Cyclin B1 in HeLa whole cell lysate ab7957 staining Cyclin B1 in HeLa whole cell lysate

References for Anti-Cyclin B1 antibody (ab7957)

This product has been referenced in:
  • Macurek L  et al. Downregulation of Wip1 phosphatase modulates the cellular threshold of DNA damage signaling in mitosis. Cell Cycle 12:251-62 (2013). Read more (PubMed: 23255129) »
  • van der Horst A & Khanna KK The peptidyl-prolyl isomerase Pin1 regulates cytokinesis through Cep55. Cancer Res 69:6651-9 (2009). WB ; Human . Read more (PubMed: 19638580) »

See all 4 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 100000 cells
Specification HeLa cells
Treatment Released from a double thymidine block
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Blocking step 2% milk and 0.5% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 20°C
Username

Dr. Kum Kum Khanna

Verified customer

Submitted Sep 07 2009

ab7957 is made with a peptide mapping to amino acids 414-433 of human origin (this is the carboxy terminus).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"