Anti-Cyclin B1 (phospho S126) antibody (ab3488)

Overview

• Product nameAnti-Cyclin B1 (phospho S126) antibodySee all Cyclin B1 primary antibodies ...
• Description
  Rabbit polyclonal to Cyclin B1 (phospho S126)
• SpecificityMinimal reactivity is expected with the non-phosphorylated form of the protein.
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Rat, Dog, Chimpanzee
• Immunogen

  Synthetic peptide: ILVDTAS_pPSPME, corresponding to amino acids 120-131 of Human phospho Cyclin B1.

  Run BLAST with Run BLAST with

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.01% Sodium Azide
  Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
• Concentration information loading...
• PurityImmunogen affinity purified
• Primary antibody notes This antibody recognizes a Ser126 phosphorylation involved in an auto phosphorylation of the cdk -cyclin complex. Recognition of this phosphorylated site suggests the antibody is useful to detect the active form of the complex and may serve as a marker of mitosis.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Cell cycle
  • Cyclins
  • Cyclin B family

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Cyclins
  • Cyclin B Family

  • Cell Biology
  • Cell Cycle
  • Cyclins
  • Cyclin B Family

Applications

Target

• FunctionEssential for the control of the cell cycle at the G2/M (mitosis) transition.
• Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
• Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
• Post-translational
  modificationsUbiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
• Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.

Target information above from: UniProt accession P14635 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 891 Human
  • Entrez Gene: 25203 Rat
  • Omim: 123836 Human
  • SwissProt: P14635 Human
  • SwissProt: P30277 Rat
  • Unigene: 23960 Human
  • Unigene: 9232 Rat

• Alternative names
  CCNB 1 antibodyCCNB antibodyCCNB1 antibody

  CCNB1_HUMAN antibodyG2 mitotic specific cyclin B1 antibodyG2/mitotic-specific cyclin-B1 antibody

  see all

Anti-Cyclin B1 (phospho S126) antibody images

• 

  Western blot - Cyclin B1 (phospho S126) antibody (ab3488)
  All lanes : Anti-Cyclin B1 (phospho S126) antibody (ab3488) at 1/100 dilution
  
  Lane 1 : HeLa whole cell lysate
  Lane 2 : H23 whole cell lysate
  Lane 3 : Hepatocarcinoma (Hep3b) cell lysate
  Lane 4 : Glioblastoma (T98G) cell lysate
  Lane 5 : B cell lymphoblast (Daudi) cell lysate
  
  Lysates/proteins at 50 µg per lane.
  
  Secondary
  HRP-conjugated Goat anti-Rabbit IgG at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 48 kDa
  
  
  Exposure time : 20 seconds