Overview

  • Product name
    Anti-Cyclin B1 (phospho S126) antibody
    See all Cyclin B1 primary antibodies
  • Description
    Rabbit polyclonal to Cyclin B1 (phospho S126)
  • Host species
    Rabbit
  • Specificity
    ab55184 detects endogenous levels of Cyclin B1 only when phosphorylated at serine 126.
  • Tested applications
    Suitable for: ICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Cyclin B1 (phospho S126). Synthetic phosphopeptide derived from human Cyclin B1 around the phosphorylation site of serine 126 (T-A-SP-P-S).
    Database link: P14635

  • Positive control
    • human lung carcinoma tissue, extracts from NIH/3T3 cells, treated with EGF.

Properties

Applications

Our Abpromise guarantee covers the use of ab55184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 19376971
WB 1/500 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 48 kDa).
ELISA 1/5000.
IHC-P 1/50 - 1/100.

Target

  • Function
    Essential for the control of the cell cycle at the G2/M (mitosis) transition.
  • Sequence similarities
    Belongs to the cyclin family. Cyclin AB subfamily.
  • Developmental stage
    Accumulates steadily during G2 and is abruptly destroyed at mitosis.
  • Post-translational
    modifications
    Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
  • Cellular localization
    Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • CCNB 1 antibody
    • CCNB antibody
    • ccnb1 antibody
    • CCNB1_HUMAN antibody
    • Cyclin B1 antibody
    • G2 mitotic specific cyclin B1 antibody
    • G2/mitotic-specific cyclin-B1 antibody
    see all

Images

  • Western blot - Cyclin B1 (phospho S126) antibody (ab55184)
    Western blot - Cyclin B1 (phospho S126) antibody (ab55184)
    All lanes : Anti-Cyclin B1 (phospho S126) antibody (ab55184) at 1/500 dilution

    Lane 1 : extracts from NIH/3T3 cells
    treated with EGF (200ng/ml, 15mins).
    Lane 2 : extracts from NIH/3T3 cells
    treated with EGF (200ng/ml, 15mins); ab36621 pre-incubated with the immunising phosphopeptide.

    Predicted band size: 48 kDa
    Observed band size: 65 kDa (why is the actual band size different from the predicted?)

  • Immunohistochemistry (Paraffin-embedded sections) - Cyclin B1 (phospho S126) antibody (ab55184)
    Immunohistochemistry (Paraffin-embedded sections) - Cyclin B1 (phospho S126) antibody (ab55184)
    Immunohistochemical analysis of Cyclin B1 (phospho S126) expression in paraffin embedded human lung carcinoma tissue using ab55184 at 1/50 dilution (left). Right hand panel represents a negative control where ab55184 was pre-incubated with the immunising (blocking) phosphopeptide.

References

This product has been referenced in:
  • Müllers E  et al. Residual Cdk1/2 activity after DNA damage promotes senescence. Aging Cell 16:575-584 (2017). Read more (PubMed: 28345297) »
  • Álvarez-Fernández M  et al. Greatwall is essential to prevent mitotic collapse after nuclear envelope breakdown in mammals. Proc Natl Acad Sci U S A 110:17374-9 (2013). Mouse . Read more (PubMed: 24101512) »

See all 4 Publications for this product

Publishing research using ab55184? Please let us know so that we can cite the reference in this datasheet.

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