Anti-Cyclin B1 (phospho S126) antibody (ab55184)

Overview

• Product nameAnti-Cyclin B1 (phospho S126) antibodySee all Cyclin B1 primary antibodies ...
• Description
  Rabbit polyclonal to Cyclin B1 (phospho S126)
• Specificityab55184 detects endogenous levels of Cyclin B1 only when phosphorylated at serine 126.
• Tested applicationsICC/IF, WB, ELISA, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Synthetic phosphopeptide derived from human Cyclin B1 around the phosphorylation site of serine 126 (T-A-S^P-P-S).

• Positive controlhuman lung carcinoma tissue, extracts from NIH/3T3 cells, treated with EGF.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThe antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Cell cycle
  • Cyclins
  • Cyclin B family

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Cyclins
  • Cyclin B Family

  • Cell Biology
  • Cell Cycle
  • Cyclins
  • Cyclin B Family

Applications

Target

• FunctionEssential for the control of the cell cycle at the G2/M (mitosis) transition.
• Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
• Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
• Post-translational
  modificationsUbiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
• Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.

Target information above from: UniProt accession P14635 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 891 Human
  • Entrez Gene: 268697 Mouse
  • Omim: 123836 Human
  • SwissProt: P14635 Human
  • SwissProt: P24860 Mouse
  • Unigene: 23960 Human
  • Unigene: 260114 Mouse
  • Unigene: 380027 Mouse

  • Unigene: 482545 Mouse

  see all

• Alternative names
  CCNB 1 antibodyCCNB antibodyCCNB1 antibody

  CCNB1_HUMAN antibodyG2 mitotic specific cyclin B1 antibodyG2/mitotic-specific cyclin-B1 antibody

  see all

Anti-Cyclin B1 (phospho S126) antibody images

• 

  Western blot - Cyclin B1 (phospho S126) antibody (ab55184)
  All lanes : Anti-Cyclin B1 (phospho S126) antibody (ab55184) at 1/500 dilution
  
  Lane 1 : extracts from NIH/3T3 cells treated with EGF (200ng/ml, 15mins).
  Lane 2 : extracts from NIH/3T3 cells treated with EGF (200ng/ml, 15mins); ab36621 pre-incubated with the immunising phosphopeptide.
  
  
  Predicted band size : 48 kDa
  Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Paraffin-embedded sections) - Cyclin B1 (phospho S126) antibody (ab55184)
  Immunohistochemical analysis of Cyclin B1 (phospho S126) expression in paraffin embedded human lung carcinoma tissue using ab55184 at 1/50 dilution (left). Right hand panel represents a negative control where ab55184 was pre-incubated with the immunising (blocking) phosphopeptide.