Anti-Cyclin B1 (phospho S126) antibody (ab55184)
- Datasheet
- Specific References (3)
- Protocols
Overview
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Product nameAnti-Cyclin B1 (phospho S126) antibody
See all Cyclin B1 primary antibodies -
DescriptionRabbit polyclonal to Cyclin B1 (phospho S126)
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Specificityab55184 detects endogenous levels of Cyclin B1 only when phosphorylated at serine 126.
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Tested applicationsSuitable for: ICC/IF, WB, ELISA, IHC-Pmore details
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Species reactivityReacts with: Mouse, Human
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Immunogen
Synthetic peptide corresponding to Human Cyclin B1 (phospho S126). Synthetic phosphopeptide derived from human Cyclin B1 around the phosphorylation site of serine 126 (T-A-SP-P-S).
Database link: P14635 -
Positive control
- human lung carcinoma tissue, extracts from NIH/3T3 cells, treated with EGF.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesThe antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunohistochemistry kits
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab55184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. PubMed: 19376971 | |
| WB | 1/500 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 48 kDa). | |
| ELISA | 1/5000. | |
| IHC-P | 1/50 - 1/100. |
Target
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FunctionEssential for the control of the cell cycle at the G2/M (mitosis) transition.
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Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
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Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
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Post-translational
modificationsUbiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases. -
Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
- Information by UniProt
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Database links
- Entrez Gene: 891 Human
- Entrez Gene: 268697 Mouse
- Omim: 123836 Human
- SwissProt: P14635 Human
- SwissProt: P24860 Mouse
- Unigene: 23960 Human
- Unigene: 260114 Mouse
- Unigene: 380027 Mouse
see all -
Alternative names
- CCNB 1 antibody
- CCNB antibody
- ccnb1 antibody
see all
Anti-Cyclin B1 (phospho S126) antibody images
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Western blot - Cyclin B1 (phospho S126) antibody (ab55184)All lanes : Anti-Cyclin B1 (phospho S126) antibody (ab55184) at 1/500 dilution
Lane 1 : extracts from NIH/3T3 cells treated with EGF (200ng/ml, 15mins).
Lane 2 : extracts from NIH/3T3 cells treated with EGF (200ng/ml, 15mins); ab36621 pre-incubated with the immunising phosphopeptide.
Predicted band size : 48 kDa
Observed band size : 65 kDa (why is the actual band size different from the predicted?) -
Immunohistochemistry (Paraffin-embedded sections) - Cyclin B1 (phospho S126) antibody (ab55184)Immunohistochemical analysis of Cyclin B1 (phospho S126) expression in paraffin embedded human lung carcinoma tissue using ab55184 at 1/50 dilution (left). Right hand panel represents a negative control where ab55184 was pre-incubated with the immunising (blocking) phosphopeptide.
Protocols
References for Anti-Cyclin B1 (phospho S126) antibody (ab55184)
This product has been referenced in:
- Álvarez-Fernández M et al. Greatwall is essential to prevent mitotic collapse after nuclear envelope breakdown in mammals. Proc Natl Acad Sci U S A 110:17374-9 (2013). Mouse . Read more (PubMed: 24101512) »
- Zheng P & Dean J Role of Filia, a maternal effect gene, in maintaining euploidy during cleavage-stage mouse embryogenesis. Proc Natl Acad Sci U S A 106:7473-8 (2009). ICC/IF ; Mouse . Read more (PubMed: 19376971) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"