Directly conjugated antibodies for immunofluorescence

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Overview

  • Product nameAnti-Cyclin B1 (phospho S126) antibody
    See all Cyclin B1 primary antibodies
  • Description
    Rabbit polyclonal to Cyclin B1 (phospho S126)
  • Specificityab55184 detects endogenous levels of Cyclin B1 only when phosphorylated at serine 126.
  • Tested applicationsICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic phosphopeptide derived from human Cyclin B1 around the phosphorylation site of serine 126 (T-A-SP-P-S).

  • Positive control
    • human lung carcinoma tissue, extracts from NIH/3T3 cells, treated with EGF.

Properties

Applications

Our Abpromise guarantee covers the use of ab55184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
ELISA
IHC-P
  • Application notesELISA: 1/5000.
    ICC/IF: Use at an assay dependent dilution (PMID 19376971).
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 48 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionEssential for the control of the cell cycle at the G2/M (mitosis) transition.
    • Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
    • Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
    • Post-translational
      modifications      Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
    • Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • CCNB 1 antibody
      • CCNB antibody
      • CCNB1 antibody
      • CCNB1_HUMAN antibody
      • G2 mitotic specific cyclin B1 antibody
      • G2/mitotic-specific cyclin-B1 antibody
      see all

    Anti-Cyclin B1 (phospho S126) antibody images

    • Western blot - Cyclin B1 (phospho S126) antibody (ab55184)
      Western blot - Cyclin B1 (phospho S126) antibody (ab55184)
      All lanes : Anti-Cyclin B1 (phospho S126) antibody (ab55184) at 1/500 dilution

      Lane 1 : extracts from NIH/3T3 cells treated with EGF (200ng/ml, 15mins).
      Lane 2 : extracts from NIH/3T3 cells treated with EGF (200ng/ml, 15mins); ab36621 pre-incubated with the immunising phosphopeptide.


      Predicted band size : 48 kDa
      Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemistry (Paraffin-embedded sections) - Cyclin B1 (phospho S126) antibody (ab55184)
      Immunohistochemistry (Paraffin-embedded sections) - Cyclin B1 (phospho S126) antibody (ab55184)
      Immunohistochemical analysis of Cyclin B1 (phospho S126) expression in paraffin embedded human lung carcinoma tissue using ab55184 at 1/50 dilution (left). Right hand panel represents a negative control where ab55184 was pre-incubated with the immunising (blocking) phosphopeptide.

    References for Anti-Cyclin B1 (phospho S126) antibody (ab55184)

    This product has been referenced in:
    • Álvarez-Fernández M  et al. Greatwall is essential to prevent mitotic collapse after nuclear envelope breakdown in mammals. Proc Natl Acad Sci U S A 110:17374-9 (2013). Mouse . Read more (PubMed: 24101512) »
    • Zheng P & Dean J Role of Filia, a maternal effect gene, in maintaining euploidy during cleavage-stage mouse embryogenesis. Proc Natl Acad Sci U S A 106:7473-8 (2009). ICC/IF ; Mouse . Read more (PubMed: 19376971) »

    See all 3 Publications for this product

    Publishing research using ab55184 ? Please let us know so that we can cite the reference in this datasheet

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