Overview

  • Product nameAnti-Cyclin B1 (phospho S126) antibodySee all Cyclin B1 primary antibodies ...
  • Description
    Rabbit polyclonal to Cyclin B1 (phospho S126)
  • Specificityab55184 detects endogenous levels of Cyclin B1 only when phosphorylated at serine 126.
  • Tested applicationsICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic phosphopeptide derived from human Cyclin B1 around the phosphorylation site of serine 126 (T-A-SP-P-S).

  • Positive control
    • human lung carcinoma tissue, extracts from NIH/3T3 cells, treated with EGF.

Properties

Applications

Our Abpromise guarantee covers the use of ab55184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
ELISA
IHC-P
  • Application notesELISA: 1/5000.
    ICC/IF: Use at an assay dependent dilution (PMID 19376971).
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 48 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionEssential for the control of the cell cycle at the G2/M (mitosis) transition.
    • Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
    • Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
    • Post-translational
      modifications
      Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
    • Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • CCNB 1 antibody
      • CCNB antibody
      • CCNB1 antibody
      • CCNB1_HUMAN antibody
      • G2 mitotic specific cyclin B1 antibody
      • G2/mitotic-specific cyclin-B1 antibody
      see all

    Anti-Cyclin B1 (phospho S126) antibody images

    • All lanes : Anti-Cyclin B1 (phospho S126) antibody (ab55184) at 1/500 dilution

      Lane 1 : extracts from NIH/3T3 cells treated with EGF (200ng/ml, 15mins).
      Lane 2 : extracts from NIH/3T3 cells treated with EGF (200ng/ml, 15mins); ab36621 pre-incubated with the immunising phosphopeptide.


      Predicted band size : 48 kDa
      Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of Cyclin B1 (phospho S126) expression in paraffin embedded human lung carcinoma tissue using ab55184 at 1/50 dilution (left). Right hand panel represents a negative control where ab55184 was pre-incubated with the immunising (blocking) phosphopeptide.

    References for Anti-Cyclin B1 (phospho S126) antibody (ab55184)

    This product has been referenced in:
    • Álvarez-Fernández M  et al. Greatwall is essential to prevent mitotic collapse after nuclear envelope breakdown in mammals. Proc Natl Acad Sci U S A 110:17374-9 (2013). Mouse . Read more (PubMed: 24101512) »
    • Zheng P & Dean J Role of Filia, a maternal effect gene, in maintaining euploidy during cleavage-stage mouse embryogenesis. Proc Natl Acad Sci U S A 106:7473-8 (2009). ICC/IF ; Mouse . Read more (PubMed: 19376971) »

    See all 3 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab55184.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"