Anti-Cyclin B1 (phospho S147) antibody (ab131502)

Overview

• Product nameAnti-Cyclin B1 (phospho S147) antibodySee all Cyclin B1 primary antibodies ...
• Description
  Rabbit polyclonal to Cyclin B1 (phospho S147)
• Specificityab131502 detects endogenous levels of Cyclin B1 only when phosphorylated at serine 147
• Tested applicationsWB, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide conjugated to KLH from around the phosphorylation site of serine 147 (AFS_pDV) of Human Cyclin B1 (NP_114172.1)

  Run BLAST with Run BLAST with
• Positive controlHeLa cells, MDA cells treated with anisomycin, Human breast carcinoma tissue.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferpH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: 48% PBS, 50% Glycerol, 0.88% Sodium chloride
  Note: PBS without Mg²⁺ and Ca²⁺
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab131502 was purified by epitope-specific phosphopeptide. Non-phosphospecific antibodies were removed by chromatography using non-phosphopeptide.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Cell cycle
  • Cyclins
  • Cyclin B family

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Cyclins
  • Cyclin B Family

  • Cell Biology
  • Cell Cycle
  • Cyclins
  • Cyclin B Family

Applications

Target

• FunctionEssential for the control of the cell cycle at the G2/M (mitosis) transition.
• Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
• Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
• Post-translational
  modificationsUbiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
• Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.

Target information above from: UniProt accession P14635 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 891 Human
  • Omim: 123836 Human
  • SwissProt: P14635 Human
  • Unigene: 23960 Human

• Alternative names
  CCNB 1 antibodyCCNB antibodyccnb1 antibody

  CCNB1_HUMAN antibodyG2 mitotic specific cyclin B1 antibodyG2/mitotic-specific cyclin-B1 antibody

  see all

Anti-Cyclin B1 (phospho S147) antibody images

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Cyclin B1 (phospho S147) antibody (ab131502)
  Immunofluorescence analysis of methanol fixed HeLa cells labelled with ab131502 at 1/100 dilution.
• 

  Western blot - Anti-Cyclin B1 (phospho S147) antibody (ab131502)
  All lanes : Anti-Cyclin B1 (phospho S147) antibody (ab131502) at 1/500 dilution
  
  Lane 1 : Untreated MDA cell lysate
  Lane 2 : Lysate of MDA cells treated with anisomycin
  
  
  Predicted band size : 48 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin B1 (phospho S147) antibody (ab131502)
  Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelled with ab131502 at 1/50 dilution (left) and ab131502 preincubated with blocking peptide (right).