Overview

  • Product nameAnti-Cyclin B1 (phospho S147) antibodySee all Cyclin B1 primary antibodies ...
  • Description
    Rabbit polyclonal to Cyclin B1 (phospho S147)
  • Tested applicationsELISA, IHC-P, WB more details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic phosphopeptide derived from human Cyclin B1 around the phosphorylation site of serine 147 (A-F-SP-D-V).

  • Positive control
    • Human placenta tissue, Jurkat cell extracts treated with UV light.

Properties

Applications

Our Abpromise guarantee covers the use of ab60986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA 1/10000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately >48 kDa (predicted molecular weight: 48 kDa).

Target

  • FunctionEssential for the control of the cell cycle at the G2/M (mitosis) transition.
  • Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
  • Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.
  • Post-translational
    modifications
    Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
  • Target information above from: UniProt accession P14635 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • CCNB 1 antibody
    • CCNB antibody
    • CCNB1 antibody
    • CCNB1_HUMAN antibody
    • G2 mitotic specific cyclin B1 antibody
    • G2/mitotic-specific cyclin-B1 antibody
    see all

Anti-Cyclin B1 (phospho S147) antibody images

  • Immunohistochemistry analysis of paraffin-embedded human placenta tissue using ab60986 at 1/50-1/100 dilution, with or without the immunising phosphopeptide.
  • All lanes : Anti-Cyclin B1 (phospho S147) antibody (ab60986) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells treated with UV light (15 mins).
    Lane 2 : Extracts from Jurkat cells treated with UV light (15 mins) and the immunising phosphopeptide.


    Predicted band size : 48 kDa
    Observed band size : >48 kDa (why is the actual band size different from the predicted?)

References for Anti-Cyclin B1 (phospho S147) antibody (ab60986)

This product has been referenced in:
  • Holt JE  et al. Spatial regulation of APCCdh1-induced cyclin B1 degradation maintains G2 arrest in mouse oocytes. Development 137:1297-304 (2010). WB ; Mouse . Read more (PubMed: 20223764) »

See 1 Publication for this product

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