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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
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Purity Immunogen affinity purified
Purification notes The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
1/500 - 1/1000. Detects a band of approximately >48 kDa (predicted molecular weight: 48 kDa).
Function Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Sequence similarities Belongs to the cyclin family. Cyclin AB subfamily.
Developmental stage Accumulates steadily during G2 and is abruptly destroyed at mitosis.
Post-translational modifications Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
Cellular localization Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome.
Information by UniProt
CCNB 1 antibody
Anti-Cyclin B1 (phospho S147) antibody images
References for Anti-Cyclin B1 (phospho S147) antibody (ab60986)
This product has been referenced in:
et al. Spatial regulation of APCCdh1-induced cyclin B1 degradation maintains G2 arrest in mouse oocytes. Development 137:1297-304 (2010).
Read more (PubMed: 20223764) »
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"