Anti-Cyclin B2/CCNB2 antibody [X121.10] (ab10839)
Key features and details
- Mouse monoclonal [X121.10] to Cyclin B2/CCNB2
- Suitable for: WB
- Reacts with: Xenopus laevis
- Isotype: IgG1
Overview
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Product name
Anti-Cyclin B2/CCNB2 antibody [X121.10]
See all Cyclin B2/CCNB2 primary antibodies -
Description
Mouse monoclonal [X121.10] to Cyclin B2/CCNB2 -
Host species
Mouse -
Specificity
ab10839 blots only cyclin B2/CCNB2, but cross reacts strongly with two other proteins. However, these can easily be distinguished from cyclins since they are present in interphase extracts and run well below the cyclins on SDS PAGE gels.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Xenopus laevis -
Immunogen
Full length native protein (purified) corresponding to Xenopus laevis Cyclin B2/CCNB2.
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Epitope
The epitope for X121 lies within a region spanning the amino acids 1-112. -
Positive control
- Xenopus egg extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
X121.10 -
Myeloma
Sp2 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10839 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa. |
Target
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Function
Essential for the control of the cell cycle at the G2/M (mitosis) transition. -
Sequence similarities
Belongs to the cyclin family. Cyclin AB subfamily. -
Developmental stage
Accumulates steadily during G2 and is abruptly destroyed at mitosis. - Information by UniProt
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Database links
- Entrez Gene: 397743 Xenopus laevis
- SwissProt: P13351 Xenopus laevis
- Unigene: 34562 Xenopus laevis
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Alternative names
- ccnb2 antibody
- CCNB2_HUMAN antibody
- CycB2 antibody
see all
Images
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All lanes : Anti-Cyclin B2/CCNB2 antibody [X121.10] (ab10839) at 1 µg/ml
Lane 1 : Interphase Xenopus egg extracts (cyclin B destroyed)
Lane 2 : Mitotic (CSF) Xenopus egg extracts (cyclin B present)
Predicted band size: 48 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Additional bands at: 38 kDa (possible cross reactivity), 40 kDa (possible cross reactivity)
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab10839 has been referenced in 5 publications.
- Li H et al. Integrative module analysis of HCC gene expression landscapes. Exp Ther Med 19:1779-1788 (2020). PubMed: 32104233
- Lin R et al. Electroacupuncture ameliorate learning and memory by improving N-acetylaspartate and glutamate metabolism in APP/PS1 mice. Biol Res 51:21 (2018). PubMed: 29980225
- Hansen DV et al. Emi2 at the crossroads: where CSF meets MPF. Cell Cycle 6:732-8 (2007). PubMed: 17361104
- Moore JD et al. Identification of the nuclear localization signal in Xenopus cyclin E and analysis of its role in replication and mitosis. Mol Biol Cell 13:4388-400 (2002). PubMed: 12475960
- Bodart JF et al. Activation of Xenopus eggs by the kinase inhibitor 6-DMAP suggests a differential regulation of cyclin B and p39(mos) proteolysis. Exp Cell Res 253:413-21 (1999). PubMed: 10585264