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Cyclin B2 protein (Tagged-His Tag) (ab91988)

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Overview

  • Product nameCyclin B2 protein (Tagged-His Tag)See all Cyclin B2 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 214-396 of Human Cyclin B2 with N terminal His tag; 183 amino acids, 21kDa (22kDa predicted from sequence). UniProt ID = O95067.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 119 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesTALLLASKYEEMFSPNIEDFVYITDNAYTSSQIREMETLI LKELKFELGRPLPLHFLRRA SKAGEVDVEQHTLAKYLM ELTLIDYDMVHYHPSKVAAAASCLSQKVLGQGKWNLKQQY YT GYTENEVLEVMQHMAKNVVKVNENLTKFIAIKNKYA SSKLLKISMIPQLNSKAVKDLASP LIG
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91988 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        ccnb2CCNB2_HUMANCycB2
        Cyclin B2G2 mitotic specific cyclin B2G2/mitotic specific cyclin B2G2/mitotic-specific cyclin-B2HsT17299MGC108931MGC140694
      see all
  • FunctionEssential for the control of the cell cycle at the G2/M (mitosis) transition.
  • Sequence similaritiesBelongs to the cyclin family. Cyclin AB subfamily.
  • Developmental stageAccumulates steadily during G2 and is abruptly destroyed at mitosis.

    • Target information above from: UniProt accession O95067 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Cyclin B2 protein (Tagged-His Tag) images

    • SDS-PAGE - Cyclin B2 protein (Tagged-His Tag) (ab91988)
      SDS-PAGE - Cyclin B2 protein (Tagged-His Tag) (ab91988)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for Cyclin B2 protein (Tagged-His Tag) (ab91988)

    ab91988 has not yet been referenced specifically in any publications.

    Publishing research using ab91988 ? Please let us know so that we can cite the reference in this datasheet

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