![Immunocytochemistry/ Immunofluorescence - Cyclin D1 antibody [DCS-6] (ab10540) image](#Cyclin-D1-Primary-antibodies-ab10540-1.jpg)
Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cell Biology >> Cell Cycle >> Cyclins >> Cyclin D Family
Overview
Product name
Anti-Cyclin D1 antibody [DCS-6]
See all Cyclin D1 products (18) ...
Description
Mouse monoclonal [DCS-6] to Cyclin D1
Specificity
Does not cross-react with other D-type cyclins.
Tested applications
ICC/IF, IHC-FoFr, WB, IHC-P, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, Monkey
Immunogen
Recombinant full length protein (Human).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Concentration
Concentration information loading...
Purity
Ascites
Clonality
Monoclonal
Clone number
DCS-6
Isotype
IgG2a
Research areas
Cancer >> Cell cycle >> Cyclins >> Cyclin D family
Epigenetics and Nuclear Signaling >> Cell cycle >> Cyclins >> Cyclin D Family
Cell Biology >> Cell Cycle >> Cyclins >> Cyclin D Family
-
Immunocytochemistry/ Immunofluorescence - Cyclin D1 antibody [DCS-6] (ab10540)
![Immunocytochemistry/ Immunofluorescence - Cyclin D1 antibody [DCS-6] (ab10540) image](/ps/datasheet/images/10/ab10540/Cyclin-D1-Primary-antibodies-ab10540-1.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10540 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
IHC-FoFr: Use at an assay dependent concentration. (PubMed: 21895533)
WB: 1/200Detects a band of approximately 36 kDa (predicted molecular weight: 33 kDa).
IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
IP: Use at an assay dependent dilution.
Target
Function
Essential for the control of the cell cycle at the G1/S (start) transition.
Involvement in disease
Note=A chromosomal aberration involving CCND1 may be a cause of B-lymphocytic malignancy, particularly mantle-cell lymphoma (MCL). Translocation t(11;14)(q13;q32) with immunoglobulin gene regions. Activation of CCND1 may be oncogenic by directly altering progression through the cell cycle.
Note=A chromosomal aberration involving CCND1 may be a cause of parathyroid adenomas. Translocation t(11;11)(q13;p15) with the parathyroid hormone (PTH) enhancer.
Defects in CCND1 are a cause of multiple myeloma (MM) [MIM:254500]. MM is a malignant tumor of plasma cells usually arising in the bone marrow and characterized by diffuse involvement of the skeletal system, hyperglobulinemia, Bence-Jones proteinuria and anemia. Complications of multiple myeloma are bone pain, hypercalcemia, renal failure and spinal cord compression. The aberrant antibodies that are produced lead to impaired humoral immunity and patients have a high prevalence of infection. Amyloidosis may develop in some patients. Multiple myeloma is part of a spectrum of diseases ranging from monoclonal gammopathy of unknown significance (MGUS) to plasma cell leukemia. Note=A chromosomal aberration involving CCND1 is found in multiple myeloma. Translocation t(11;14)(q13;q32) with the IgH locus.
Sequence similarities
Belongs to the cyclin family. Cyclin D subfamily.
Post-translational
modifications
Phosphorylation at Thr-286 by MAP kinases is required for ubiquitination and degradation following DNA damage. It probably plays an essential role for recognition by the FBXO31 component of SCF (SKP1-cullin-F-box) protein ligase complex.
Ubiquitinated, primarily as 'Lys-48'-linked polyubiquitination. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex containing FBXO4 and CRYAB (By similarity). Following DNA damage it is ubiquitinated by some SCF (SKP1-cullin-F-box) protein ligase complex containing FBXO31. Ubiquitination leads to its degradation and G1 arrest. Deubiquitinated by USP2; leading to stabilize it.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP24385
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AI327039 antibody
- B cell ccl/lymphoma 1 antibody
- B cell CLL/lymphoma 1 antibody
see all
Database links
- Entrez Gene: 595 Human
- Entrez Gene: 12443 Mouse
- Entrez Gene: 58919 Rat
- Omim: 168461 Human
- SwissProt: P24385 Human
- SwissProt: P25322 Mouse
- SwissProt: P39948 Rat
- Unigene: 523852 Human
see all
Anti-Cyclin D1 antibody [DCS-6] images:
Immunocytochemistry/ Immunofluorescence - Cyclin D1 antibody [DCS-6] (ab10540)
ICC/IF image of ab10540 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10540, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Cyclin D1 antibody [DCS-6] (ab10540)
This product has been referenced in:
- Kabadi SVet al. Cyclin D1 Gene Ablation Confers Neuroprotection in Traumatic Brain Injury. J Neurotrauma : (2011). IHC-FoFr; Mouse.Read more (PubMed: 21895533) »
- Wang XDet al. SUMO-modified nuclear cyclin D1 bypasses Ras-induced senescence. Cell Death Differ : (2010). WB, ICC/IF, IP; Human.Read more (PubMed: 20798689) »
See all 3 publications for this product
Publishing research using ab10540? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"