Western blot abreview for Anti-Cyclin D1 antibody [EPR2241]

Excellent
Abreviews
Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (13%)
Sample
Human Cell lysate - whole cell (HeLa and MEF cell lysates)
Specification
HeLa and MEF cell lysates
Treatment
See ab136811
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% Milk in PBS-T buffer
Dilution
1/5000

Secondary antibody

Name
Dilution
1/10000

Detection

Detection method
ECL
Bands
Specific: 34 kDa
Exposure
5 second(s)

Additional data

Additional Notes
HeLa cell cycle lysates (ab136811) were used to check cell cycle dependent expression of Cyclin D1. Antibody works also in murine cells.
Username

Mr. Christian Marx

Verified customer

Submitted Dec 04 2013

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