Western blot abreview for Anti-Cyclin D1 antibody [EPR2241]

Excellent
Abreviews
Application Western blot
Loading amount 15 µg
Gel Running Conditions Reduced Denaturing (13%)
Sample Human Cell lysate - whole cell (HeLa and MEF cell lysates)
Specification HeLa and MEF cell lysates
Treatment See ab136811
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time 16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% Milk in PBS-T buffer
Dilution 1/5000

Secondary antibody

Name Abcam antibody:Goat Anti-Rabbit IgG H&L (HRP)
Dilution 1/10000

Detection

Detection method ECL
Bands Specific: 34 kDa
Exposure 5 second(s)

Additional data

Additional Notes HeLa cell cycle lysates (ab136811) were used to check cell cycle dependent expression of Cyclin D1. Antibody works also in murine cells.
Username

Mr. Christian Marx

Verified customer

Submitted Dec 04 2013