Immunocytochemistry/ Immunofluorescence abreview for Anti-Cyclin D1 antibody [EPR2241]

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.5% Triton X100 in PBS
Fixative
Paraformaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/200

Secondary antibody

Secondary antibody
None used

Additional data

Additional Notes
Asynchronous cells were paraformaldehyde fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. This antibody produces the expected cytoplasmic & nuclear staining pattern and is enriched within G1 cells. MeOH fixed samples were also evaluated and produced a similar, albeit slightly weaker staining pattern.
Username

Dr. Kirk Mcmanus

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Submitted Jan 22 2014

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