Immunocytochemistry/ Immunofluorescence abreview for Anti-Cyclin D1 antibody [EPR2241]

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Permeabilization Yes - 0.5% Triton X100 in PBS
Fixative Paraformaldehyde

Other product details

Incubation time 1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution 1/200

Secondary antibody

Secondary antibody None used

Additional data

Additional Notes Asynchronous cells were paraformaldehyde fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. This antibody produces the expected cytoplasmic & nuclear staining pattern and is enriched within G1 cells. MeOH fixed samples were also evaluated and produced a similar, albeit slightly weaker staining pattern.

Dr. Kirk Mcmanus

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Submitted Jan 22 2014