Western blot abreview for Anti-Cyclin D1 antibody [EPR2241]

Application Western blot
Loading amount 50000 cells
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (MCF-7, MDA-MB-231)
Specification MCF-7, MDA-MB-231
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time 16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBS
Dilution 1/2000

Secondary antibody

Name Non-Abcam antibody was used: Goat anti-rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution 1/2000


Detection method ECL
Bands Specific: 30-40 kDa
Exposure 5 minute(s) and 0 second(s)
Positive control MCF-7, MDA-MB-231

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Submitted Aug 04 2014