Immunocytochemistry/ Immunofluorescence abreview for Anti-Cyclin D1 antibody [EPR2241]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample
Human Cell (MCF-7)
Specification
MCF-7
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% goat serum+0.1% BSA+0.1% Triton+PBS
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor 488 goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500
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Submitted Aug 04 2014

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