Western blot abreview for Anti-Cyclin D1 antibody [EPR2241]

Excellent
Abreviews
Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - whole (Heart)
Specification
Heart
Blocking step
superblock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C

Other product details

Incubation time
3 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Goat-Anti-Rabbit-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000

Detection

Detection method
ECL
Bands
Specific: 34 kDa
Exposure
20 second(s)
Positive control
Heart Tissue
Username

Abcam user community

Verified customer

Submitted Aug 25 2014

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