Western blot abreview for Anti-Cyclin D1 antibody [EPR2241]

Application Western blot
Loading amount 15 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Tissue lysate - whole (Heart)
Specification Heart
Blocking step superblock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C

Other product details

Incubation time 3 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution 1/1000

Secondary antibody

Name Non-Abcam antibody was used: Goat-Anti-Rabbit-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution 1/20000


Detection method ECL
Bands Specific: 34 kDa
Exposure 20 second(s)
Positive control Heart Tissue

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Submitted Aug 25 2014