Western blot abreview for Anti-Cyclin D1 antibody [EPR2241]

Excellent
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (osteosarcoma cells)
Specification
osteosarcoma cells
Treatment
lane 1 control, lane 2 siRNA for Cyclin D1, 48h
Blocking step
Milk as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 21°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% BSA TBS-T
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbitt
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
ECL
Bands
Specific: 34 kDa
Exposure
5 second(s)
Negative control
lane 2 - siRNA for Cyclin D1.
Positive control
lane 1 - control, non-targeting siRNA.
Username

Dr. Sonia Rocha

Verified customer

Submitted Sep 26 2014

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