Western blot abreview for Anti-Cyclin D1 antibody [EPR2241]

Average
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Human Epithelial OVCAR cell line)
Gel Running Conditions
Reduced Denaturing (10 % SDS-PAGE)
Loading amount
100 µg
Treatment
Perifosine 0-24 hr
Specification
Human Epithelial OVCAR cell line
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% milk
Dilution
1/1000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL Western Blotting Substrate (Femto)
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 35 kDa Non-specific: 30, 45 kDa
Username

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Verified customer

Submitted Apr 20 2017

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