Products:Cell Biology >> Cell Cycle >> Cyclins >> Cyclin D Family
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ab16663 has been referenced in 13 publications.
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Lane 1 : Anti-Cyclin D1 antibody [SP4] (ab16663) at 1/200 dilution
Lane 2 : Anti-Cyclin D1 antibody [SP4] (ab16663) at 1/400 dilution
Lane 1 : Whole cell lysate prepared from T24 bladder cancer cells
Lane 2 : Whole cell lysate prepared from T24 bladder cancer cells
Lysates/proteins at 25 µg per lane.
Secondary
Goat anti-rabbit IgG conjugated to HRP at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 33 kDa
Observed band size : 33 kDa
Exposure time : 10 minutes
Gel run under denaturing conditions 4-12% gradient.
Image kindly supplied by Dr Karin Birkenkamp-Demtroeder through Abreview
ab16663 staining Cyclin D1 in Human urinary tract tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% BSA for 30 minutes at room temperature; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/100 in PBS) for 1 hour. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
This image is courtesy of an Abreview submitted by Karin Birkenkamp-Demtroeder
Anti-Cyclin D1 antibody [SP4] (ab16663) at 1/25 dilution + MCF7 cell lysate
Predicted band size : 33 kDa
Observed band size : 33 kDa
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