Products:Cell Biology >> Cell Cycle >> Cyclins >> Cyclin D Family
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I see this antibody is not purified and is in tissue culture supernatant? Is the tissue culture supernatant serum free? |
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ANSWER: |
ab16663 is serum free. I hope this is helpful. Please do not hesitate to contact me with any additional questions. |
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Please provide the concentration of this antibody. |
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ANSWER: |
Thank you for your telephone enquiry yesterday regarding antibody ab16663. I can confirm that the concentration of this antibody is around 0.1mg/ml. However,as discussed on the phone, the antibody is supplied as cell culture supernatant which means the concentration will vary slightly and this is an approximate value. I hope this information is helpful. Should you have any further questions, please do not hesitate to contact us again. |
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Thanks Adam. Is that 70% homology for the immunogenic peptide then, or the whole protein? Tom |
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ANSWER: |
Thank you for your enquiry. The homology of the peptide used to generate ab16663 shows ~70% homology with both cyclin D2 and D3. There is a possibility of cross-reaction, but we do not have data to confirm it. Please let me know if you have additional questions. |
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Could you tell me the isotype of this rabbit monoclonal antibody or the specificity of secondary antibody to use for
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ANSWER: |
Thank you for your enquiry. Ab16663 is a rabbit IgG antibody, so you should use an anti-rabbit IgG secondary antibody. At the bottom of the online datasheet there is also a list of compatible secondaries which can be used. If you have any additional questions, please contact us again. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Lane 1 : Anti-Cyclin D1 antibody [SP4] (ab16663) at 1/200 dilution
Lane 2 : Anti-Cyclin D1 antibody [SP4] (ab16663) at 1/400 dilution
Lane 1 : Whole cell lysate prepared from T24 bladder cancer cells
Lane 2 : Whole cell lysate prepared from T24 bladder cancer cells
Lysates/proteins at 25 µg per lane.
Secondary
Goat anti-rabbit IgG conjugated to HRP at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 33 kDa
Observed band size : 33 kDa
Exposure time : 10 minutes
Gel run under denaturing conditions 4-12% gradient.
Image kindly supplied by Dr Karin Birkenkamp-Demtroeder through Abreview
ab16663 staining Cyclin D1 in Human urinary tract tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% BSA for 30 minutes at room temperature; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/100 in PBS) for 1 hour. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
This image is courtesy of an Abreview submitted by Karin Birkenkamp-Demtroeder
Anti-Cyclin D1 antibody [SP4] (ab16663) at 1/25 dilution + MCF7 cell lysate
Predicted band size : 33 kDa
Observed band size : 33 kDa
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