Cyclin D1 antibody [SP4] (ab16663)

Product Name 

Cyclin D1 antibody [SP4]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [SP4] to Cyclin D1

Immunogen 

Synthetic peptide (Human) (C terminal).

Reacts with 

Hu, Ms

Predicted to react 
(species key)

Rat
(Due to sequence homology)

Epitope 

C-terminus

Tested applications 
(see key)

ICC/IF, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at an assay dependent dilution.
IHC-P: 1/100. Staining of formalin-fixed tissues is required by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
WB: 1/200. Predicted molecular weight: 33 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Breast carcinomas

Cellular localization 

Nuclear

Research areas 

Cancer >> Cell cycle >> Cyclins >> Cyclin D family
Chromatin and Nuclear Signaling >> Cell cycle >> Cyclins >> Cyclin D Family
Cell Biology >> Cell Cycle >> Cyclins >> Cyclin D Family

Relevance 

During each cell cycle cyclins undergo periodic accumulation and destruction. As key regulators of the cell cycle the cyclins control important transitions by acting as regulatory subunits of the Cdks. Early in the G1 phase of the cell cycle, cyclin D1 induction is followed by cyclin E induction. This sequential progression is marked early on in G1 by the activation of Cdk4 and in mid to late G1 by the activation of Cdk2 and the hyperphosphorylation of pRB. The final transition into S phase is thought to be dependent on the increased expression and association of cyclin E and Cdk2.
In a recent study, Cyclin D1 regulates cellular metabolism, fat cell differentiation and cellular migration. Cyclin D1 is also involved in development and cancer.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

SP4

Isotype 

IgG

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.1% Sodium Azide

Form 

Liquid

Concentration 

0.100 mg/ml

Storage instructions 

Store at +4°C.

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References for Cyclin D1 antibody [SP4] (ab16663)

  This product has been used in: (two most recent references)

Ogino S et al. A cohort study of cyclin d1 expression and prognosis in 602 colon cancer cases. Clin Cancer Res 15:4431-8 (2009). IHC-P; Human. PubMed: 19549773

Genander M et al. Dissociation of EphB2 signaling pathways mediating progenitor cell proliferation and tumor suppression. Cell 139:679-92 (2009). WB; Mouse. PubMed: 19914164

See all 10 publication references for this product.


If you publish research using ab16663 please let us know so that we can cite the reference on this datasheet.

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