![- Cyclin D1 antibody [SP4] (ab16663) image](#abcam1-16663.jpg)
![Western blot - Cyclin D1 antibody [SP4] (ab16663) image](#Cyclin-D1-Primary-antibodies-ab16663-2.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cyclin D1 antibody [SP4] (ab16663) image](#Cyclin-D1-Primary-antibodies-ab16663-4.jpg)
![- Cyclin D1 antibody [SP4] (ab16663)](/ps/datasheet/Images/16/ab16663/abcam1-16663.jpg)
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Products:Cell Biology >> Cell Cycle >> Cyclins >> Cyclin D Family
Overview
Product name
Anti-Cyclin D1 antibody [SP4]
See all Cyclin D1 products (18) ...
Description
Rabbit monoclonal [SP4] to Cyclin D1
Tested applications
ICC/IF, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat
Immunogen
Synthetic peptide (Human) (C terminal).
Epitope
C-terminus
Positive control
Breast carcinomas
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
SP4
Isotype
IgG
Research areas
Cancer >> Cell cycle >> Cyclins >> Cyclin D family
Epigenetics and Nuclear Signaling >> Cell cycle >> Cyclins >> Cyclin D Family
Cell Biology >> Cell Cycle >> Cyclins >> Cyclin D Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab16663 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution.
WB: 1/200Predicted molecular weight: 33 kDa.
IHC-P: 1/100
Target
Function
Essential for the control of the cell cycle at the G1/S (start) transition.
Involvement in disease
Note=A chromosomal aberration involving CCND1 may be a cause of B-lymphocytic malignancy, particularly mantle-cell lymphoma (MCL). Translocation t(11;14)(q13;q32) with immunoglobulin gene regions. Activation of CCND1 may be oncogenic by directly altering progression through the cell cycle.
Note=A chromosomal aberration involving CCND1 may be a cause of parathyroid adenomas. Translocation t(11;11)(q13;p15) with the parathyroid hormone (PTH) enhancer.
Defects in CCND1 are a cause of multiple myeloma (MM) [MIM:254500]. MM is a malignant tumor of plasma cells usually arising in the bone marrow and characterized by diffuse involvement of the skeletal system, hyperglobulinemia, Bence-Jones proteinuria and anemia. Complications of multiple myeloma are bone pain, hypercalcemia, renal failure and spinal cord compression. The aberrant antibodies that are produced lead to impaired humoral immunity and patients have a high prevalence of infection. Amyloidosis may develop in some patients. Multiple myeloma is part of a spectrum of diseases ranging from monoclonal gammopathy of unknown significance (MGUS) to plasma cell leukemia. Note=A chromosomal aberration involving CCND1 is found in multiple myeloma. Translocation t(11;14)(q13;q32) with the IgH locus.
Sequence similarities
Belongs to the cyclin family. Cyclin D subfamily.
Post-translational
modifications
Phosphorylation at Thr-286 by MAP kinases is required for ubiquitination and degradation following DNA damage. It probably plays an essential role for recognition by the FBXO31 component of SCF (SKP1-cullin-F-box) protein ligase complex.
Ubiquitinated, primarily as 'Lys-48'-linked polyubiquitination. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex containing FBXO4 and CRYAB (By similarity). Following DNA damage it is ubiquitinated by some SCF (SKP1-cullin-F-box) protein ligase complex containing FBXO31. Ubiquitination leads to its degradation and G1 arrest. Deubiquitinated by USP2; leading to stabilize it.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP24385
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AI327039 antibody
- B cell ccl/lymphoma 1 antibody
- B cell CLL/lymphoma 1 antibody
see all
Database links
- Entrez Gene: 595 Human
- Entrez Gene: 12443 Mouse
- Entrez Gene: 58919 Rat
- Omim: 168461 Human
- SwissProt: P24385 Human
- SwissProt: P25322 Mouse
- SwissProt: P39948 Rat
- Unigene: 523852 Human
see all
Anti-Cyclin D1 antibody [SP4] images:
Western blot - Cyclin D1 antibody [SP4] (ab16663)
![Western blot - Cyclin D1 antibody [SP4] (ab16663)](/ps/datasheet/images/16/ab16663/Cyclin-D1-Primary-antibodies-ab16663-2.jpg)
Lane 1 : Anti-Cyclin D1 antibody [SP4] (ab16663) at 1/200 dilution
Lane 2 : Anti-Cyclin D1 antibody [SP4] (ab16663) at 1/400 dilution
Lane 1 : Whole cell lysate prepared from T24 bladder cancer cells
Lane 2 : Whole cell lysate prepared from T24 bladder cancer cells
Lysates/proteins at 25 µg per lane.
Secondary
Goat anti-rabbit IgG conjugated to HRP at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 33 kDa
Observed band size : 33 kDa
Exposure time : 10 minutes
Gel run under denaturing conditions 4-12% gradient.
Image kindly supplied by Dr Karin Birkenkamp-Demtroeder through Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cyclin D1 antibody [SP4] (ab16663)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cyclin D1 antibody [SP4] (ab16663)](/ps/datasheet/images/16/ab16663/Cyclin-D1-Primary-antibodies-ab16663-4.jpg)
ab16663 staining Cyclin D1 in Human urinary tract tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% BSA for 30 minutes at room temperature; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/100 in PBS) for 1 hour. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
This image is courtesy of an Abreview submitted by Karin Birkenkamp-Demtroeder
Western blot - Cyclin D1 antibody [SP4] (ab16663)
![Western blot - Cyclin D1 antibody [SP4] (ab16663)](/ps/datasheet/images/16/ab16663/Cyclin-D1-Primary-antibodies-ab16663-5.jpg)
Anti-Cyclin D1 antibody [SP4] (ab16663) at 1/25 dilution + MCF7 cell lysate
Predicted band size : 33 kDa
Observed band size : 33 kDa
References for Anti-Cyclin D1 antibody [SP4] (ab16663)
This product has been referenced in:
- Read MLet al. Proto-oncogene PBF/PTTG1IP regulates thyroid cell growth and represses radioiodide treatment. Cancer Res 71:6153-64 (2011). IHC-P; Mouse.Read more (PubMed: 21844185) »
- Almeida MQet al. Mouse Prkar1a haploinsufficiency leads to an increase in tumors in the Trp53+/- or Rb1+/- backgrounds and chemically induced skin papillomas by dysregulation of the cell cycle and Wnt signaling. Hum Mol Genet 19:1387-98 (2010). IHC-P; Mouse.Read more (PubMed: 20080939) »
See all 12 publications for this product
Publishing research using ab16663? Please let us know so that we can cite the reference in this datasheet
![Cyclin D1 antibody [SP4] for WB in Human (16663)](/ps/datasheet/images/16/ab16663/Cyclin-D1-Primary-antibodies-ab16663-1.jpg)
![Cyclin D1 antibody [SP4] for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Human (16663)](/ps/datasheet/images/16/ab16663/Cyclin-D1-Primary-antibodies-ab16663-3.jpg)
![Cyclin D1 antibody [SP4] for ICC/IF in Human (16663)](/ps/Reviews/Images/ab11433_62049.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"