Anti-Cyclin D1 antibody (ab75717)

Overview

• Product nameAnti-Cyclin D1 antibodySee all Cyclin D1 primary antibodies ...
• Description
  Rabbit polyclonal to Cyclin D1
• Tested applicationsIHC-P, WB, ELISA more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  A KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S90 of human Cyclin D1.

• Positive controlMouse lung and bladder tissue lysates

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThis antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Cell cycle
  • Cyclins
  • Cyclin D family

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Cyclins
  • Cyclin D Family

  • Cell Biology
  • Cell Cycle
  • Cyclins
  • Cyclin D Family

Applications

Target

• FunctionEssential for the control of the cell cycle at the G1/S (start) transition.
• Involvement in diseaseNote=A chromosomal aberration involving CCND1 may be a cause of B-lymphocytic malignancy, particularly mantle-cell lymphoma (MCL). Translocation t(11;14)(q13;q32) with immunoglobulin gene regions. Activation of CCND1 may be oncogenic by directly altering progression through the cell cycle.
  Note=A chromosomal aberration involving CCND1 may be a cause of parathyroid adenomas. Translocation t(11;11)(q13;p15) with the parathyroid hormone (PTH) enhancer.
  Defects in CCND1 are a cause of multiple myeloma (MM) [MIM:254500]. MM is a malignant tumor of plasma cells usually arising in the bone marrow and characterized by diffuse involvement of the skeletal system, hyperglobulinemia, Bence-Jones proteinuria and anemia. Complications of multiple myeloma are bone pain, hypercalcemia, renal failure and spinal cord compression. The aberrant antibodies that are produced lead to impaired humoral immunity and patients have a high prevalence of infection. Amyloidosis may develop in some patients. Multiple myeloma is part of a spectrum of diseases ranging from monoclonal gammopathy of unknown significance (MGUS) to plasma cell leukemia. Note=A chromosomal aberration involving CCND1 is found in multiple myeloma. Translocation t(11;14)(q13;q32) with the IgH locus.
• Sequence similaritiesBelongs to the cyclin family. Cyclin D subfamily.
• Post-translational
  modificationsPhosphorylation at Thr-286 by MAP kinases is required for ubiquitination and degradation following DNA damage. It probably plays an essential role for recognition by the FBXO31 component of SCF (SKP1-cullin-F-box) protein ligase complex.
  Ubiquitinated, primarily as 'Lys-48'-linked polyubiquitination. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex containing FBXO4 and CRYAB (By similarity). Following DNA damage it is ubiquitinated by some SCF (SKP1-cullin-F-box) protein ligase complex containing FBXO31. Ubiquitination leads to its degradation and G1 arrest. Deubiquitinated by USP2; leading to stabilize it.
• Cellular localizationNucleus.

Target information above from: UniProt accession P24385 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 595 Human
  • Entrez Gene: 12443 Mouse
  • Omim: 168461 Human
  • SwissProt: P24385 Human
  • SwissProt: P25322 Mouse
  • Unigene: 523852 Human
  • Unigene: 667996 Human
  • Unigene: 273049 Mouse

• Alternative names
  AI327039 antibodyB cell CLL/lymphoma 1 antibodyB cell leukemia 1 antibody

  B cell lymphoma 1 protein antibodyB-cell lymphoma 1 protein antibodyBCL 1 antibodyBCL-1 antibodyBCL-1 oncogene antibodyBCL1 antibodyBCL1 oncogene antibodyccnd1 antibodyCCND1/FSTL3 fusion gene, included antibodyCCND1/IGHG1 fusion gene, included antibodyCCND1/IGLC1 fusion gene, included antibodyCCND1/PTH fusion gene, included antibodyCCND1_HUMAN antibodycD1 antibodyCyl 1 antibodyD11S287E antibodyG1/S specific cyclin D1 antibodyG1/S-specific cyclin-D1 antibodyParathyroid adenomatosis 1 antibodyPRAD1 antibodyPRAD1 oncogene antibodyU21B31 antibody

  see all

Anti-Cyclin D1 antibody images

• 

  Western blot - Cyclin D1 antibody (ab75717)
  All lanes : Anti-Cyclin D1 antibody (ab75717) at 1/50 dilution
  
  Lane 1 : mouse lung tissue lysate
  Lane 2 : mouse bladder tissue lysate
  
  Lysates/proteins at 35 µg per lane.
  
  
  Predicted band size : 33 kDa
  Observed band size : 33 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cyclin D1 antibody (ab75717)
  ab75717, at a dilution of 1/10, staining Cyclin D1 in formalin fixed, paraffin embedded human breast carcinoma tissue by Immunohistochemistry. ab75717 was peroxidase conjugated to the secondary antibody, followed by DAB staining.