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Overview

  • Product nameAnti-Cyclin D1 antibodySee all Cyclin D1 primary antibodies ...
  • Description
    Rabbit polyclonal to Cyclin D1
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Chinese Hamster, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Human Cyclin D1.

    Read Abcam's proprietary immunogen policy

  • Positive controlThis antibody gave a positive signal in the following whole cell lysates: T24/83; MCF7; SHSY-5Y; U-87 MG.

Properties

Applications

Our Abpromise guarantee covers the use of ab95281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).

Target

  • FunctionEssential for the control of the cell cycle at the G1/S (start) transition.
  • Involvement in diseaseNote=A chromosomal aberration involving CCND1 may be a cause of B-lymphocytic malignancy, particularly mantle-cell lymphoma (MCL). Translocation t(11;14)(q13;q32) with immunoglobulin gene regions. Activation of CCND1 may be oncogenic by directly altering progression through the cell cycle.
    Note=A chromosomal aberration involving CCND1 may be a cause of parathyroid adenomas. Translocation t(11;11)(q13;p15) with the parathyroid hormone (PTH) enhancer.
    Defects in CCND1 are a cause of multiple myeloma (MM) [MIM:254500]. MM is a malignant tumor of plasma cells usually arising in the bone marrow and characterized by diffuse involvement of the skeletal system, hyperglobulinemia, Bence-Jones proteinuria and anemia. Complications of multiple myeloma are bone pain, hypercalcemia, renal failure and spinal cord compression. The aberrant antibodies that are produced lead to impaired humoral immunity and patients have a high prevalence of infection. Amyloidosis may develop in some patients. Multiple myeloma is part of a spectrum of diseases ranging from monoclonal gammopathy of unknown significance (MGUS) to plasma cell leukemia. Note=A chromosomal aberration involving CCND1 is found in multiple myeloma. Translocation t(11;14)(q13;q32) with the IgH locus.
  • Sequence similaritiesBelongs to the cyclin family. Cyclin D subfamily.
  • Post-translational
    modifications    Phosphorylation at Thr-286 by MAP kinases is required for ubiquitination and degradation following DNA damage. It probably plays an essential role for recognition by the FBXO31 component of SCF (SKP1-cullin-F-box) protein ligase complex.
    Ubiquitinated, primarily as 'Lys-48'-linked polyubiquitination. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex containing FBXO4 and CRYAB (By similarity). Following DNA damage it is ubiquitinated by some SCF (SKP1-cullin-F-box) protein ligase complex containing FBXO31. Ubiquitination leads to its degradation and G1 arrest. Deubiquitinated by USP2; leading to stabilize it.
  • Cellular localizationNucleus.

    • Target information above from: UniProt accession P24385 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      AI327039 antibodyB cell CLL/lymphoma 1 antibodyB cell leukemia 1 antibody
      B cell lymphoma 1 protein antibodyB-cell CLL/lymphoma 1 antibodyB-cell leukemia 1 antibodyB-cell lymphoma 1 protein antibodyBCL 1 antibodyBCL-1 antibodyBCL-1 oncogene antibodyBCL1 antibodyBCL1 oncogene antibodyCCND1 antibodyCCND1/FSTL3 fusion gene, included antibodyCCND1/IGHG1 fusion gene, included antibodyCCND1/IGLC1 fusion gene, included antibodyCCND1/PTH fusion gene, included antibodyCCND1_HUMAN antibodycD1 antibodyCyclin D1 antibodyCyl 1 antibodyCyl-1 antibodyD11S287E antibodyG1/S specific cyclin D1 antibodyG1/S-specific cyclin-D1 antibodyMGC137744 antibodyParathyroid adenomatosis 1 antibodyPRAD1 antibodyPRAD1 oncogene antibodyU21B31 antibody
    see all

Anti-Cyclin D1 antibody images

  • Western blot - Cyclin D1 antibody (ab95281)
    Western blot - Cyclin D1 antibody (ab95281)
    All lanes : Anti-Cyclin D1 antibody (ab95281) at 1 µg/ml

    Lane 1 : T24/83 (Human bladder carcinoma) Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 4 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 34 kDa
    Additional bands at : 140 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes

References for Anti-Cyclin D1 antibody (ab95281)

ab95281 has not yet been referenced specifically in any publications.

Publishing research using ab95281 ? Please let us know so that we can cite the reference in this datasheet

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