You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Cyclin D2 293T Transfected Lysate - (positive control) (ab94090)

Submit a Question

Overview

  • Product nameCyclin D2 293T Transfected Lysate - (positive control)
  • General notesab94090 is a 293T cell transfected lysate in which Human Cyclin D2 has been transiently over-expressed using a pCMV-Cyclin D2 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsWB more details

Properties

  • BackgroundFunction: Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Similarity: Belongs to the cyclin family. Cyclin D subfamily. Contains 1 cyclin N-terminal domain.

    • Applications

    Our Abpromise guarantee covers the use of ab94090 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB
  • Application notesWB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Cyclin D2 293T Transfected Lysate - (positive control) images

    • SDS-PAGE - Cyclin D2 293T Transfected Lysate - (positive control) (ab94090)
      SDS-PAGE - Cyclin D2 293T Transfected Lysate - (positive control) (ab94090)
      ab94090 at 15µg/lane on an SDS-PAGE gel.
    • Western blot - Cyclin D2 293T Transfected Lysate - (positive control) (ab94090)
      Western blot - Cyclin D2 293T Transfected Lysate - (positive control) (ab94090)
      All lanes : Anti-Cyclin D2 antibody (ab89595) at 1/500 dilution

      Lane 1 : Cyclin D2 293T Transfected Lysate - (positive control) (ab94090)
      Lane 2 : 293T non-transfected lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

    References for Cyclin D2 293T Transfected Lysate - (positive control) (ab94090)

    ab94090 has not yet been referenced specifically in any publications.

    Publishing research using ab94090 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit a Question for ab94090

    There are currently no Questions for ab94090.
    Please use the link above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"