Anti-Cyclin D2 antibody (ab79482)

Overview

  • Product nameAnti-Cyclin D2 antibody
    See all Cyclin D2 primary antibodies
  • Description
    Rabbit polyclonal to Cyclin D2
  • Specificityab79482 detects endogenous levels of total Cyclin D2 protein
  • Tested applicationsWB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic non-phosphopeptide derived from human Cyclin D2 around the phosphorylation site of threonine 280 (ASTPPT)

  • Positive control
    • Exracts from 293 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab79482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 33 kDa.
ELISA 1/20000.

Target

  • FunctionRegulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
  • Sequence similaritiesBelongs to the cyclin family. Cyclin D subfamily.
    Contains 1 cyclin N-terminal domain.
  • Cellular localizationNucleus. Cytoplasm. Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members.
  • Information by UniProt
  • Database links
  • Alternative names
    • CCND 2 antibody
    • CCND2 antibody
    • CCND2_HUMAN antibody
    • CyclinD2 antibody
    • G1/S specific cyclin D2 antibody
    • G1/S-specific cyclin-D2 antibody
    • KIAK0002 antibody
    • MGC102758 antibody
    see all

Anti-Cyclin D2 antibody images

  • All lanes : Anti-Cyclin D2 antibody (ab79482) at 1/1000 dilution

    Lane 1 : extracts from 293 cells
    Lane 2 : extracts from 293 cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 33 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)

References for Anti-Cyclin D2 antibody (ab79482)

ab79482 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. The immunogen sequence of this antibody is from amino acid 239 to 289. As you have mentioned in your previous email that the test sequence only contain ASTPPT sequence so I am afraid this antibody will not work with the...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"