Anti-Cyclin D2 antibody (ab79482)

Overview

• Product nameAnti-Cyclin D2 antibodySee all Cyclin D2 primary antibodies ...
• Description
  Rabbit polyclonal to Cyclin D2
• Specificityab79482 detects endogenous levels of total Cyclin D2 protein
• Tested applicationsWB, ELISA more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat
• Immunogen

  Synthetic non-phosphopeptide derived from human Cyclin D2 around the phosphorylation site of threonine 280 (AST^PPT)

• Positive controlExracts from 293 cells

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Cell cycle
  • Cyclins
  • Cyclin D family

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Cyclins
  • Cyclin D Family

  • Cell Biology
  • Cell Cycle
  • Cyclins
  • Cyclin D Family

Applications

Target

• FunctionRegulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
• Sequence similaritiesBelongs to the cyclin family. Cyclin D subfamily.
  Contains 1 cyclin N-terminal domain.
• Cellular localizationNucleus. Cytoplasm. Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members.

Target information above from: UniProt accession P30279 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 894 Human
  • Entrez Gene: 12444 Mouse
  • Entrez Gene: 64033 Rat
  • Omim: 123833 Human
  • SwissProt: P30279 Human
  • SwissProt: P30280 Mouse
  • SwissProt: Q04827 Rat
  • Unigene: 376071 Human

  • Unigene: 333406 Mouse
  • Unigene: 96083 Rat

  see all

• Alternative names
  CCND 2 antibodyCCND2 antibodyCCND2_HUMAN antibody

  CyclinD2 antibodyG1/S specific cyclin D2 antibodyG1/S-specific cyclin-D2 antibodyKIAK0002 antibodyMGC102758 antibody

  see all

Anti-Cyclin D2 antibody images

• 

  Western blot - Cyclin D2 antibody (ab79482)
  All lanes : Anti-Cyclin D2 antibody (ab79482) at 1/1000 dilution
  
  Lane 1 : extracts from 293 cells
  Lane 2 : extracts from 293 cells with immunising peptide at 10 µg
  
  Lysates/proteins at 30 µg per lane.
  
  
  Predicted band size : 33 kDa
  Observed band size : 43 kDa (why is the actual band size different from the predicted?)