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Anti-Cyclin E antibody [EP435E] (ab33911)

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Overview

Product name

Anti-Cyclin E antibody [EP435E]
See all Cyclin E products (22) ...

Description

Rabbit monoclonal [EP435E] to Cyclin E

Specificity

This antibody recognises Cyclin E. It is predicted to detect the splice isoform 2 based on sequence analysis.

Tested applications

WB, ICC/IF, Flow Cyt, IPmore details

Cross reactivity

Reacts with

Human

Does not react with

Mouse, Rat

Immunogen

A synthetic peptide corresponding to residues in human Cyclin E.

Positive control

HeLa cells and cell lysate.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

EP435E

Isotype

IgG

Research areas

Cancer >> Cell cycle >> Cyclins >> Cyclin E family
Epigenetics and Nuclear Signaling >> Cell cycle >> Cyclins >> Cyclin E Family
Cell Biology >> Cell Cycle >> Cyclins >> Cyclin E Family
Cell Biology >> Cell Cycle >> Cyclins

  • Western blot - Cyclin E antibody [EP435E] (ab33911)Western blot - Cyclin E antibody [EP435E] (ab33911) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - Cyclin E antibody [EP435E] (ab33911)Immunocytochemistry/ Immunofluorescence - Cyclin E antibody [EP435E] (ab33911) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab33911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Is unsuitable for or IHC.

Target

Function

Essential for the control of the cell cycle at the G1/S (start) transition.

Tissue specificity

Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.

Sequence similarities

Belongs to the cyclin family. Cyclin E subfamily.

Post-translational
modifications

Phosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR.

Cellular localization

Nucleus.

Target information above from: UniProt accessionP24864 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CCNE 1 antibody
  • CCNE antibody
  • CCNE1 antibody
  • CCNE1_HUMAN antibody
  • Cyclin E1 antibody
  • Cyclin Es antibody
  • Cyclin Et antibody
  • CyclinE antibody
  • G1/S specific cyclin E antibody
  • G1/S-specific cyclin-E1 antibody
see all

Database links

Anti-Cyclin E antibody [EP435E] images:

  Western blot - Cyclin E antibody [EP435E] (ab33911)

Western blot - Cyclin E antibody [EP435E] (ab33911)

Anti-Cyclin E antibody [EP435E] (ab33911) at 1/2000 dilution + HeLa cell lysate

Predicted band size : 47 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)

  Immunocytochemistry/ Immunofluorescence - Cyclin E antibody [EP435E] (ab33911)

Immunocytochemistry/ Immunofluorescence - Cyclin E antibody [EP435E] (ab33911)

References for Anti-Cyclin E antibody [EP435E] (ab33911)

ab33911 has not yet been referenced specifically in any publications.

Publishing research using ab33911? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"