Anti-Cyclin E1 antibody (ab71535)
Key features and details
- Rabbit polyclonal to Cyclin E1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Cyclin E1 antibody
See all Cyclin E1 primary antibodies -
Description
Rabbit polyclonal to Cyclin E1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human Cyclin E1.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.03% Sodium azide
Constituents: 0.01% BSA, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, HEPES -
Concentration information loading...
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Purity
IgG fraction -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab71535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 47 kDa).
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Notes |
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WB
1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 47 kDa). |
Target
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Function
Essential for the control of the cell cycle at the G1/S (start) transition. -
Tissue specificity
Highly expressed in testis and placenta. Low levels in bronchial epithelial cells. -
Sequence similarities
Belongs to the cyclin family. Cyclin E subfamily. -
Post-translational
modificationsPhosphorylation of Thr-395 by GSK3 and of Ser-399 by CDK2 accelerates degradation via the ubiquitin proteasome pathway. Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 898 Human
- Entrez Gene: 12447 Mouse
- Entrez Gene: 25729 Rat
- Omim: 123837 Human
- SwissProt: P24864 Human
- SwissProt: Q61457 Mouse
- SwissProt: P39949 Rat
- Unigene: 244723 Human
see all -
Alternative names
- CCNE antibody
- Ccne1 antibody
- CCNE1_HUMAN antibody
see all
Images
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All lanes : Anti-Cyclin E1 antibody (ab71535) at 1/2000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Mouse liver lysate
Lane 4 : Rat liver lysate
Predicted band size: 47 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (25)
ab71535 has been referenced in 25 publications.
- Zheng X et al. TAK1 accelerates transplant arteriosclerosis in rat aortic allografts by inducing autophagy in vascular smooth muscle cells. Atherosclerosis 343:10-19 (2022). PubMed: 35078016
- Jeong JH & Ryu JH Urushiol V Suppresses Cell Proliferation and Enhances Antitumor Activity of 5-FU in Human Colon Cancer Cells by Downregulating FoxM1. Biomol Ther (Seoul) 30:257-264 (2022). PubMed: 35264465
- Zhou Q et al. Integrated systemic analysis of FAM72A to identify its clinical relevance, biological function, and relationship to drug sensitivity in hepatocellular carcinoma. Front Oncol 12:1046473 (2022). PubMed: 36483027
- Lei Y et al. HBx induces hepatocellular carcinogenesis through ARRB1-mediated autophagy to drive the G1/S cycle. Autophagy 17:4423-4441 (2021). PubMed: 33866937
- Roy N et al. mRNP granule proteins Fmrp and Dcp1a differentially regulate mRNP complexes to contribute to control of muscle stem cell quiescence and activation. Skelet Muscle 11:18 (2021). PubMed: 34238354