You have changed your country from to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab2098 for help.
Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.
|
|||||||||
 | |||||||||
|
|||||||||
BATCH NUMBER 115294 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM more bands, unspecific SAMPLE Cell extract PRIMARY ANTIBODY Abcam, ab2098,Rabbit polyclonal 1:1000, 1h RT, wash 5 times a 5 min with Western wash DETECTION METHOD ECL POSITIVE AND NEGATIVE CONTROLS USED no, but other batches from you always worked fine ANTIBODY STORAGE CONDITIONS aliquoted a 20ul in -20 SAMPLE PREPARATION Ripa Buffer, Protese Inhibotors AMOUNT OF PROTEIN LOADED 20-50ug ELECTROPHORESIS/GEL CONDITIONS SDS-PAGE 10% TRANSFER AND BLOCKING CONDITIONS 1 hour blocking in 5% BSA in Western Wash Buffer SECONDARY ANTIBODY Jackson Immuno Research :Goat anti rabbit IgG-HRP 1:10.000, 1 hr, RT HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 20 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? nothing altered, since we got fine results with other batch (we are out of this Antidody)
|
|||||||||
ANSWER: |
Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. The problems that you have been experiencing definitely appear to be batch related. I am certainly prepared to offer you a credit note against this purchase provided that the antibody was purchased within the past 90 days. If this is the case please email me details of the order including the order number and date of purchase. I look forward to hearing from you. |
||||||||
|
|||||||||
Customer's enquiry: Is the immunogen sequence available? |
|||||||||
ANSWER: |
Sorry, the sequence is not available. |
||||||||
|
|||||||||
Is 85 mg/ml correct? |
|||||||||
ANSWER: |
The protein concentration is 85 mg/ml. |
||||||||
|
|||||||||
I am interested in using either cyclin T1-ab2098 or cdk9-ab6544 in a chromatin immunoprecipitation assay using human B cells to identify binding by P-TEFb (PITALRE) in vivo. Have either antibody been used in this assay and which would you recommend trying of the two? Many thanks Dr. Julian knight |
|||||||||
ANSWER: |
Neither of these antibodies has yet been tested for ChIP, although they work very well in standard IP. We are unable to suggest which of the two may be better. However, we would be very interested to hear the results and will reward you with an amazon.com gift certificate ($30) for you data. Good luck with your experiments, |
||||||||
|
|||||||||
| |||||||||
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
