Cyclin T1 antibody (ab27963)

Product Name 

Cyclin T1 antibody

Product type 

Primary antibodies

Description 

Sheep polyclonal to Cyclin T1

Immunogen 

Recombinant fragment, corresponding to C terminal amino acids 291-726 of Human Cyclin T1

Reacts with 

Hu, Ms, Hrs, Mk

Does not react with 
(species key)

Dm

Tested applications 
(see key)

EMSA, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
EMSA: Use at an assay dependent dilution PMID 17576689.
IP: Use at an assay dependent dilution.
WB: 1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 81 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Cell cycle >> Cyclins >> Other Cyclins
Cell Biology >> Cell Cycle >> Cyclins >> Other Cyclins
Microbiology >> Interspecies Interaction >> Host Virus Interaction

Relevance 

Cyclin T1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. This cyclin tightly associates with CDK9 kinase, and was found to be a major subunit of the transcription elongation factor p-TEFb. The kinase complex containing this cyclin and the elongation factor can interact with, and act as a cofactor of human immunodeficiency virus type 1 (HIV-1) Tat protein, and was shown to be both necessary and sufficient for full activation of viral transcription. This cyclin and its kinase partner were also found to be involved in the phosphorylation and regulation of the carboxy-terminal domain (CTD) of the largest RNA polymerase II subunit.

ab27963 will also recognise truncated forms of cyclin T1 which are frequently produced by proteolysis during sample preparation.

Database links 

Raised in 

Sheep

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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References for Cyclin T1 antibody (ab27963)

  This product has been used in: (two most recent references)

Krueger BJ et al. LARP7 is a stable component of the 7SK snRNP while P-TEFb, HEXIM1 and hnRNP A1 are reversibly associated. Nucleic Acids Res : (2008). WB; Human. PubMed: 18281698

Sedore SC et al. Manipulation of P-TEFb control machinery by HIV: recruitment of P-TEFb from the large form by Tat and binding of HEXIM1 to TAR. Nucleic Acids Res 35:4347-58 (2007). EMSA; Human. PubMed: 17576689
If you publish research using ab27963 please let us know so that we can cite the reference on this datasheet.

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