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Anti-Cyclin T1 antibody (ab27963)

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Overview

Product name

Anti-Cyclin T1 antibody
See all Cyclin T1 products (4) ...

Description

Sheep polyclonal to Cyclin T1

Tested applications

WB, IP, EMSAmore details

Cross reactivity

Reacts with

Mouse, Horse, Human, Monkey

Does not react with

Fruit fly (Drosophila melanogaster)

Immunogen

Recombinant fragment, corresponding to C terminal amino acids 291-726 of Human Cyclin T1

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Purity

Whole antiserum

Primary antibody notes

ab27963 will also recognise truncated forms of cyclin T1 which are frequently produced by proteolysis during sample preparation.

Clonality

Polyclonal

Isotype

IgG

Research areas

Immunology >> Immune System Diseases >> Antiviral Signaling >> HIV-related
Epigenetics and Nuclear Signaling >> Cell cycle >> Cyclins >> Other Cyclins
Cell Biology >> Cell Cycle >> Cyclins >> Other Cyclins
Microbiology >> Interspecies Interaction >> Host Virus Interaction

  • Western blot - Cyclin T1 antibody (ab27963)Western blot - Cyclin T1 antibody (ab27963) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab27963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

EMSA: Use at an assay dependent dilution PMID 17576689.
IP: Use at an assay dependent dilution.
WB: 1/1000. Detects a band of approximately 87 kDa (predicted molecular weight: 81 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes.

Tissue specificity

Ubiquitously expressed.

Sequence similarities

Belongs to the cyclin family. Cyclin C subfamily.

Cellular localization

Nucleus.

Target information above from: UniProt accessionO60563 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • CCN T1 antibody
  • CCNT antibody
  • CCNT 1 antibody
  • CCNT1 antibody
  • CCNT1_HUMAN antibody
  • CDK9 associated C type protein antibody
  • Cyc T1 antibody
  • Cyclin C related protein antibody
  • cyclin T antibody
  • cyclin T1 antibody
  • Cyclin T1b antibody
  • Cyclin-T antibody
  • cyclin-T1 antibody
  • CYCT 1 antibody
  • cycT1 antibody
  • HIVE1 antibody
  • Human immunodeficiency virus 1 expression antibody
  • Human immunodeficiency virus type 1 (HIV 1) expression (elevated) 1 antibody
  • pTEFb subunit antibody
  • Subunit of positive elongation transcription factor b antibody
see all

Database links

see all

Anti-Cyclin T1 antibody images:

  Western blot - Cyclin T1 antibody (ab27963)

Western blot - Cyclin T1 antibody (ab27963)

All lanes : Anti-Cyclin T1 antibody (ab27963) at 1/1000 dilution

Lane 1 : HeLa whole cell lysate
Lane 2 : HeLa cell nuclear lysate
Lane 3 : monkey Vero cell lysate
Lane 4 : mouse kidney cell lysate
Lane 5 : Drosophila S2 cell lysate
Lane 6 : horse dermal cell lysate

Secondary
donkey anti-sheep peroxidase conjugate at 1/20000 dilution

Predicted band size : 81 kDa
Observed band size : 87 kDa (why is the actual band size different from the predicted?)

References for Anti-Cyclin T1 antibody (ab27963)

This product has been referenced in:

  • Krueger BJet al. LARP7 is a stable component of the 7SK snRNP while P-TEFb, HEXIM1 and hnRNP A1 are reversibly associated. Nucleic Acids Res : (2008). WB; Human.Read more (PubMed: 18281698) »
  • Sedore SCet al. Manipulation of P-TEFb control machinery by HIV: recruitment of P-TEFb from the large form by Tat and binding of HEXIM1 to TAR. Nucleic Acids Res 35:4347-58 (2007). EMSA; Human.Read more (PubMed: 17576689) »

See all 2 publications for this product

Publishing research using ab27963? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"