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Products:Microbiology >> Interspecies Interaction >> Host Virus Interaction
Overview
Product name
Anti-Cyclin T1 antibody
See all Cyclin T1 products (4) ...
Description
Rabbit polyclonal to Cyclin T1
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Dog, Pig, Chimpanzee, Zebrafish
Immunogen
Synthetic peptide corresponding to a region within N terminal amino acids 2 - 51 (EGERKNNNKR WYFTREQLEN SPSRRFGVDP DKELSYRQQA ANLLQDMGQR) of human Cyclin T1 (NP_001231).
EGERKNNNKR WYFTREQLEN SPSRRFGVDP DKELSYRQQA ANLLQDMGQR
Positive control
721_B cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab83314 is purified by a peptide affinity chromatography method.
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Immune System Diseases >> Antiviral Signaling >> HIV-related
Epigenetics and Nuclear Signaling >> Cell cycle >> Cyclins >> Other Cyclins
Cell Biology >> Cell Cycle >> Cyclins >> Other Cyclins
Microbiology >> Interspecies Interaction >> Host Virus Interaction
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Western blot - Cyclin T1 antibody (ab83314)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab83314 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: Use at an assay dependent dilution.
ELISA titre using peptide based assay, 1:1562500
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 81 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes.
Tissue specificity
Ubiquitously expressed.
Sequence similarities
Belongs to the cyclin family. Cyclin C subfamily.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO60563
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CCN T1 antibody
- CCNT antibody
- CCNT 1 antibody
see all
Database links
- Entrez Gene: 904 Human
- Entrez Gene: 12455 Mouse
- Entrez Gene: 315291 Rat
- Entrez Gene: 334465 Zebrafish
- Omim: 143055 Human
- SwissProt: Q6T8E9 Cow
- SwissProt: O60563 Human
- SwissProt: Q9QWV9 Mouse
see all
Anti-Cyclin T1 antibody images:
Western blot - Cyclin T1 antibody (ab83314)
Anti-Cyclin T1 antibody (ab83314) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 81 kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Additional bands at : 45 kDa,88 kDa. We are unsure as to the identity of these extra bands.
References for Anti-Cyclin T1 antibody (ab83314)
ab83314 has not yet been referenced specifically in any publications.
Publishing research using ab83314? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"