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Western blot - Cyclin T1 antibody (ab83314)
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Product Name
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Cyclin T1 antibody
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See all Cyclin T1 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Cyclin T1
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Immunogen
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Synthetic peptide corresponding to a region within N terminal amino acids 2 - 51 (EGERKNNNKR WYFTREQLEN SPSRRFGVDP DKELSYRQQA ANLLQDMGQR) of human Cyclin T1 (NP_001231).
BLAST 'EGERKNNNKR WYFTREQLEN SPSRRFGVDP DKELSYRQQA ANLLQDMGQR ' with  or 
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
ELISA titre using peptide based assay, 1:1562500
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 81 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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721_B cell lysate
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Cell cycle >> Cyclins >> Other Cyclins
Cell Biology >> Cell Cycle >> Cyclins >> Other Cyclins
Microbiology >> Interspecies Interaction >> Host Virus Interaction
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Relevance
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Cyclin T1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. This cyclin tightly associates with CDK9 kinase, and was found to be a major subunit of the transcription elongation factor p-TEFb. The kinase complex containing this cyclin and the elongation factor can interact with, and act as a cofactor of human immunodeficiency virus type 1 (HIV-1) Tat protein, and was shown to be both necessary and sufficient for full activation of viral transcription. This cyclin and its kinase partner were also found to be involved in the phosphorylation and regulation of the carboxy-terminal domain (CTD) of the largest RNA polymerase II subunit.
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Cyclin T1 antibody (ab83314) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Purification notes
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ab83314 is purified by a peptide affinity chromatography method.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Cyclin T1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Cyclin T1 antibody images, references, products related to ab83314 and other tools.
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Cyclin T1 antibody images:
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 Western blot - Cyclin T1 antibody (ab83314)
Cyclin T1 antibody (ab83314) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 81 kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Additional bands at : 45 kDa,88 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to Cyclin T1
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Cyclin T1 antibody - more information
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Customer reviews (feedback) regarding Cyclin T1 antibody |
Customer FAQs regarding Cyclin T1 antibody |
Protocols for Cyclin T1 antibody |
Price and availability of products related to Cyclin T1 antibody |
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Cyclin T1 antibody - related products:
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Cyclin T1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Cyclin T1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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