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Product name
Anti-Cyclophilin 40 antibody
See all Cyclophilin 40 products (5) ...
Description
Rabbit polyclonal to Cyclophilin 40
Specificity
Detects cyclophilin 40 (CyP 40) from human and rat tissues and cells. This antibody does not cross-react with CyPA.
Tested applications
Flow Cyt, Gel supershift assays, ICC, IHC-Fr, IP, WBmore details
Cross reactivity
Reacts with
Rat, Human
Immunogen
Synthetic peptide: C-AQKDKEKAVYAKMFA, corresponding to amino acids 356-370 of Human Cyclophilin 40.
C-AQKDKEKAVYAKMFA
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: PBS
Purity
Whole antiserum
Primary antibody notes
Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents: cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. CyP 40, a 40 kDa protein, shares significant homology with smaller CyPA (CyP 18) and FKBP59. CyP 40 exhibits the characteristic CsA binding and isomerase activity of CyP 18, though these activities appear to be less with CyP 40 than with Cyp 18. Like FKBP59, CyP 40 has been found in progesterone receptor complexes. CyP 40 is expressed at similar levels in many tissues.
Clonality
Polyclonal
Isotype
IgG
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use at an assay dependent dilution.
GSA: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
Target
Function
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Tissue specificity
Widely expressed.
Sequence similarities
Belongs to the cyclophilin-type PPIase family. PPIase D subfamily.
Contains 1 PPIase cyclophilin-type domain.
Contains 3 TPR repeats.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ08752
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 40 kDa peptidyl prolyl cis trans isomerase antibody
- 40 kDa peptidyl prolyl cis trans isomerase D antibody
- 40 kDa peptidyl-prolyl cis-trans isomerase antibody
see all
Database links
- Entrez Gene: 5481 Human
- Entrez Gene: 5481 Human
- Entrez Gene: 361967 Rat
- Entrez Gene: 361967 Rat
- Omim: 601753 Human
- SwissProt: Q08752 Human
- SwissProt: Q08752 Human
- SwissProt: Q6DGG0 Rat
see all
Anti-Cyclophilin 40 antibody images:
Western blot - Cyclophilin 40 antibody (ab3562)
ab3562 at a dilution of 1/1000 staining Cyp 40 in rat spleen lysate by Western blot.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin 40 antibody (ab3562)
Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized human hepatocarcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at aab3562) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin 40 antibody (ab3562)
Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized human skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH 6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1/100 with a rabbit polyclonal antibody recognizing Cyclophilin D (ab3562) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
References for Anti-Cyclophilin 40 antibody (ab3562)
This product has been referenced in:
- Han J et al. Deregulation of mitochondrial membrane potential by mitochondrial insertion of granzyme B and direct hax-1 cleavage. J Biol Chem 285:22461-72 (2010). WB; Human.Read more (PubMed: 20388708) »
- Trauernicht AM et al. Modulation of estrogen receptor alpha protein level and survival function by DBC-1. Mol Endocrinol 21:1526-36 (2007).Read more (PubMed: 17473282) »
See all 2 publications for this product
Publishing research using ab3562? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"