Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-Cyclophilin A antibody
See all Cyclophilin A products (8) ...
Description
Rabbit polyclonal to Cyclophilin A
Specificity
Detects recombinant human Cyclophilin A (CyPA), but does not detect endogenous levels of CyPA.
Tested applications
ICC/IF, WB, ELISAmore details
Cross reactivity
Reacts with
Rabbit, Human
Immunogen
Purified recombinant human CyPA expressed in E-coli.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS with 0.05% sodium azide
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Signaling Pathway >> Calcium Signaling >> Other
Immunology >> Immune Interventions >> Transplantation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3563 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml.
WB: 1/300.
ELISA: Use at an assay dependent dilution. Used at a dilution of 1/1000 for 2 hrs on rabbit liver cells (see Abreview).
Target
Function
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Sequence similarities
Belongs to the cyclophilin-type PPIase family. PPIase A subfamily.
Contains 1 PPIase cyclophilin-type domain.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP62937
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cyclophilin A antibody
- Cyclophilin antibody
- CyclophilinA antibody
see all
Database links
- Entrez Gene: 5478 Human
- Omim: 123840 Human
- SwissProt: Q567Q0 Human
- SwissProt: P62937 Human
- SwissProt: Q9TTC6 Rabbit
- Unigene: 356331 Human
- Unigene: 598115 Human
Anti-Cyclophilin A antibody images:
Western blot - Anti-Cyclophilin A antibody (ab3563)
ab3563 detecting 0.5ug recombinant human cyclophilin A by Western Blot.
Immunocytochemistry/ Immunofluorescence-Cyclophilin A antibody(ab3563)
ICC/IF image of ab3563 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3563, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Cyclophilin A antibody (ab3563)
This product has been referenced in:
- Lorenson MY et al. A major prolactin-binding complex on human milk fat globule membranes contains cyclophilins A and B: the complex is not the prolactin receptor. Am J Physiol Endocrinol Metab 302:E585-94 (2012). WB; Human.Read more (PubMed: 22205628) »
- Waller H et al. The use of AlphaLISA technology to detect interaction between hepatitis C virus-encoded NS5A and cyclophilin A. J Virol Methods 165:202-10 (2010).Read more (PubMed: 20132841) »
See all 4 publications for this product
Publishing research using ab3563? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"