Overview
- Product nameAnti-Cystatin C antibodySee all Cystatin C primary antibodies ...
- DescriptionGoat polyclonal to Cystatin C
- Tested applicationsWB more details
- Species reactivityReacts with: Mouse
- Immunogen
Synthetic peptide: GTHSLTKFSCKNA, corresponding to C terminal amino acids 128-140 of mouse Cystatin C(Peptide available as ab22916.)
- Positive controlMouse kidney lysate. Mouse testis lysate.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... - PurityIgG fraction
- Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab2516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 16 kDa).Can be blocked with Cystatin C peptide (128-140) (ab22916). |
Target
- FunctionAs an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
- Tissue specificityExpressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland.
- Involvement in diseaseAmyloidosis 6 (AMYL6) [MIM:105150]: A hereditary generalized amyloidosis due to cystatin C amyloid deposition. Cystatin C amyloid accumulates in the walls of arteries, arterioles, and sometimes capillaries and veins of the brain, and in various organs including lymphoid tissue, spleen, salivary glands, and seminal vesicles. Amyloid deposition in the cerebral vessels results in cerebral amyloid angiopathy, cerebral hemorrhage and premature stroke. Cystatin C levels in the cerebrospinal fluid are abnormally low. Note=The disease is caused by mutations affecting the gene represented in this entry.
Age-related macular degeneration 11 (ARMD11) [MIM:611953]: A form of age-related macular degeneration, a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry. - Sequence similaritiesBelongs to the cystatin family.
- Post-translational
modificationsThe Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21. - Cellular localizationSecreted.
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Database links
- Entrez Gene: 13010 Mouse
- SwissProt: P21460 Mouse
- Unigene: 4263 Mouse
Target information above from: UniProt accession
P01034
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- AD 8 antibodyAD8 antibodyAmyloid angiopathy and cerebral hemorrhage antibody
- ARMD11 antibodybA218C14.4 (cystatin C) antibodybA218C14.4 antibodyCst 3 antibodyCst3 antibodyCST3 protein antibodyCystatin 3 antibodyCystatin-3 antibodyCystatin-C antibodyCystatin3 antibodyCystatinC antibodyCYTC_HUMAN antibodyGamma trace antibodyGamma-trace antibodyHCCAA antibodyMGC117328 antibodyNeuroendocrine basic polypeptide antibodyPost gamma globulin antibodyPost-gamma-globulin antibody
see all
Anti-Cystatin C antibody images
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Western blot - Cystatin C antibody (ab2516)Anti-Cystatin C antibody (ab2516) at 0.01 µg/ml + mouse testis lysate in RIPA buffer at 35 µg
developed using the ECL technique
Predicted band size : 16 kDa
Observed band size : 18 kDa (why is the actual band size different from the predicted?)
Primary incubated for 1 hour.
References for Anti-Cystatin C antibody (ab2516)
ab2516 has not yet been referenced specifically in any publications.
