Anti-Cystatin C antibody (ab2516)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab2516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 16 kDa).Can be blocked with Human Cystatin C peptide (ab22916).

Target

  • FunctionAs an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
  • Tissue specificityExpressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland.
  • Involvement in diseaseAmyloidosis 6
    Age-related macular degeneration 11
  • Sequence similaritiesBelongs to the cystatin family.
  • Post-translational
    modifications
    The Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21.
  • Cellular localizationSecreted.
  • Target information above from: UniProt accession P01034 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • AD 8 antibody
    • AD8 antibody
    • Amyloid angiopathy and cerebral hemorrhage antibody
    • ARMD11 antibody
    • bA218C14.4 (cystatin C) antibody
    • bA218C14.4 antibody
    • Cst 3 antibody
    • Cst3 antibody
    • CST3 protein antibody
    • Cystatin 3 antibody
    • Cystatin-3 antibody
    • Cystatin-C antibody
    • Cystatin3 antibody
    • CystatinC antibody
    • CYTC_HUMAN antibody
    • Gamma trace antibody
    • Gamma-trace antibody
    • HCCAA antibody
    • MGC117328 antibody
    • Neuroendocrine basic polypeptide antibody
    • Post gamma globulin antibody
    • Post-gamma-globulin antibody
    see all

Anti-Cystatin C antibody images

  • Anti-Cystatin C antibody (ab2516) at 0.01 µg/ml + mouse testis lysate in RIPA buffer at 35 µg
    developed using the ECL technique

    Predicted band size : 16 kDa
    Observed band size : 18 kDa (why is the actual band size different from the predicted?)


    Primary incubated for 1 hour.

References for Anti-Cystatin C antibody (ab2516)

ab2516 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (MDA MB 231 cells)
Loading amount 10 µg
Specification MDA MB 231 cells
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

Submitted Apr 14 2009

Thank you for your enquiry. I am sorry to hear you are having a problem with ab2516. As we monitor all complaints on an antibody, we do troubleshoot the problem first to determine whether the cause is protocol-related or due to the antibody not funct...

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Thank you for your enquiry. We regret to inform you that we can't provide this antibody in PBS buffer. We would suggest dialyse the product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"