Anti-Cystatin C antibody (ab85790)
- Product nameAnti-Cystatin C antibodySee all Cystatin C primary antibodies ...
- DescriptionRabbit polyclonal to Cystatin C
- Tested applicationsICC/IF, WB, IP, Sandwich ELISA, IHC-P more details
- Species reactivityReacts with: Human
Human recombinant Cystatin C.
- Positive controlHepG2 cell line Human lung FFPE tissue sections
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4
- Concentration information loading...
- PurityImmunogen affinity purified
- Purification notesab85790 is purified by immunoaffinity, utilizing human recombinant Cystatin C agarose as an affinity matrix
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab85790 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||ICC/IF: Use a concentration of 1 µg/ml.|
|WB||WB: Use at an assay dependent dilution. Predicted molecular weight: 16 kDa.|
|IP||IP: Use at an assay dependent dilution.|
|Sandwich ELISA||sELISA: Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [Cyst-13] to Cystatin C (ab24327). For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with Ab24327 as Capture.|
|IHC-P||IHC-P: Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
- FunctionAs an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.
- Tissue specificityExpressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland.
- Involvement in diseaseAmyloidosis 6 (AMYL6) [MIM:105150]: A hereditary generalized amyloidosis due to cystatin C amyloid deposition. Cystatin C amyloid accumulates in the walls of arteries, arterioles, and sometimes capillaries and veins of the brain, and in various organs including lymphoid tissue, spleen, salivary glands, and seminal vesicles. Amyloid deposition in the cerebral vessels results in cerebral amyloid angiopathy, cerebral hemorrhage and premature stroke. Cystatin C levels in the cerebrospinal fluid are abnormally low. Note=The disease is caused by mutations affecting the gene represented in this entry.
Age-related macular degeneration 11 (ARMD11) [MIM:611953]: A form of age-related macular degeneration, a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
- Sequence similaritiesBelongs to the cystatin family.
modificationsThe Thr-25 variant is O-glycosylated with a core 1 or possibly core 8 glycan. The signal peptide of the O-glycosylated Thr-25 variant is cleaved between Ala-20 and Val-21.
- Cellular localizationSecreted.
- AD 8 antibodyAD8 antibodyAmyloid angiopathy and cerebral hemorrhage antibody
- ARMD11 antibodybA218C14.4 (cystatin C) antibodybA218C14.4 antibodyCst 3 antibodyCst3 antibodyCST3 protein antibodyCystatin 3 antibodyCystatin-3 antibodyCystatin-C antibodyCystatin3 antibodyCystatinC antibodyCYTC_HUMAN antibodyGamma trace antibodyGamma-trace antibodyHCCAA antibodyMGC117328 antibodyNeuroendocrine basic polypeptide antibodyPost gamma globulin antibodyPost-gamma-globulin antibody
Anti-Cystatin C antibody images
Standard Curve for Cystatin C (Analyte: ab7653) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [Cyst-13] to Cystatin C (ab24327) at 1ug/ml and Detector Antibody Rabbit polyclonal to Cystatin C (ab85790) at 0.5ug/ml
ICC/IF image of ab85790 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85790, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of Cystatin C staining in human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85790, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-Cystatin C antibody (ab85790)
ab85790 has not yet been referenced specifically in any publications.