Anti-Cystatin S antibody (ab151771)
Key features and details
- Rabbit polyclonal to Cystatin S
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cystatin S antibody -
Description
Rabbit polyclonal to Cystatin S -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide, corresponding to a region within amino acids 1-65 of Human Cystatin S (UniProt P01036).
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Positive control
- 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates, A431 cells, SW480 xenograft
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151771 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 16 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) is also possible.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 16 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) is also possible. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
This protein strongly inhibits papain and ficin, partially inhibits stem bromelain and bovine cathepsin C, but does not inhibit porcine cathepsin B or clostripain. Papain is inhibited non-competitively. -
Tissue specificity
Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in saliva, tears, urine and seminal fluid. -
Sequence similarities
Belongs to the cystatin family. -
Post-translational
modificationsPhosphorylated at both its N- and C-terminal regions. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 1472 Human
- Omim: 123857 Human
- SwissProt: P01036 Human
- Unigene: 654549 Human
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Alternative names
- Cst4 antibody
- Cystatin 4 antibody
- Cystatin S precursor antibody
see all
Images
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Anti-Cystatin S antibody (ab151771) at 1/1000 dilution + HepG2 whole cell
lysate at 30 µg
Predicted band size: 16 kDa -
Immunofluorescent analysis of methanol-fixed A431 cells labeling Cystatin S with ab151771 at 1/200. Lower panel co-stained with Hoechst 33342.
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Immunohistochemical analysis of paraffin embedded SW480 xenograft, labeling Cystatin S with ab151771 antibody at 1/100.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab151771 has been referenced in 2 publications.
- Odanaka H et al. Comparison of protein profiles of the pellicle, gingival crevicular fluid, and saliva: possible origin of pellicle proteins. Biol Res 53:3 (2020). PubMed: 31941552
- Jain S et al. Salivary proteome patterns of individuals exposed to High Altitude. Arch Oral Biol 96:104-112 (2018). PubMed: 30219637