CytoPainter F-actin Staining Kit - Orange Fluorescence (ab112126)
- Product nameCytoPainter F-actin Staining Kit - Orange FluorescenceSee all F-actin kits ...
- Sample typeCell culture extracts, Tissue, Adherent cells, Suspension cells
- Assay typeCell-based
- Product overview
Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.
ab112126 is designed to stain F-actins in fixed cells with orange fluorescence. ab112126 uses an orange fluorescent phalloidin conjugate that is selectively bound to F-actins. The orange fluorescent phalloidin conjugate has Ex/Em = 550/575 nm. The conjugate also has good photostability for fluorescence imaging. It is a high-affinity probe for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. ab112126 provides all the essential components with an optimized labeling protocol.
ab112126 should be stored dessicated.
- Tested applicationsFluorescence Microscopy, ICC/IF more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components 500 tests Labeling Buffer 1 x 50ml Orange Fluorescent Phalloidin Conjugate 1 x 50µl
- Research Areas
Our Abpromise guarantee covers the use of ab112126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Fluorescence Microscopy||Fluorescence Microscopy|
CytoPainter F-actin Staining Kit - Orange Fluorescence images
ab112126, staining CPA cells fixed with formaldehyde in a black 96-well plate.
Left panel: The cells were labeled with Orange Fluorescent Phalloidin Conjugate for 30 minutes only.
Right panel: The cells were treated with phalloidin for 10 minutes, then stained with Orange Fluorescent Phalloidin Conjugate for 30 minutes.
References for CytoPainter F-actin Staining Kit - Orange Fluorescence (ab112126)
ab112126 has not yet been referenced specifically in any publications.