CytoPainter F-actin Staining Kit - Orange Fluorescence (ab112126)

Overview

  • Product nameCytoPainter F-actin Staining Kit - Orange FluorescenceSee all F-actin kits ...
  • Sample type
    Cell culture extracts, Tissue, Adherent cells, Suspension cells
  • Assay typeCell-based
  • Product overview

    Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.

    ab112126 is designed to stain F-actins in fixed cells with orange fluorescence. ab112126 uses an orange fluorescent phalloidin conjugate that is selectively bound to F-actins. The orange fluorescent phalloidin conjugate has Ex/Em = 550/575 nm. The conjugate also has good photostability for fluorescence imaging. It is a high-affinity probe for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. ab112126 provides all the essential components with an optimized labeling protocol.

  • Notes

    ab112126 should be stored dessicated.

  • Tested applicationsFluorescence Microscopy, ICC/IF more details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab112126 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Fluorescence Microscopy Use at an assay dependent concentration.
    ICC/IF Use at an assay dependent concentration.

    CytoPainter F-actin Staining Kit - Orange Fluorescence images

    • ab112126, staining CPA cells fixed with formaldehyde in a black 96-well plate.
      Left panel: The cells were labeled with Orange Fluorescent Phalloidin Conjugate for 30 minutes only.
      Right panel: The cells were treated with phalloidin for 10 minutes, then stained with Orange Fluorescent Phalloidin Conjugate for 30 minutes.

    Protocols

    References for CytoPainter F-actin Staining Kit - Orange Fluorescence (ab112126)

    ab112126 has not yet been referenced specifically in any publications.

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    The CytoPainter kits are compatible with secondary antibodies and nuclear dyes for multicolor staining or GFP-transfected cells, for example. The lab has confirmed that these kits can be used with other antibodies...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"