CytoPainter Lysosomal Staining Kit - Green Fluorescence (ab112136)
- Product nameCytoPainter Lysosomal Staining Kit - Green FluorescenceSee all Lysosome kits ...
- Tests1 x 500 test
- Sample typeAdherent cells, Suspension cells
- Assay typeCell-based
- Product overview
Abcam's fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as lysosomes, mitochondria, and actin filaments. The selective staining of cell compartments provides a powerful method for studying cellular events in a spatial and temporal context.
ab112136 is designed to label lysosomes in live cells with green fluorescence at Ex/Em = 490/525 nm. The proprietary lysotropic dye used in the kit selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and is trapped in lysosomes after it gets into cells. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly reduces its staining background and makes it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.
- Tested applicationsFluorescence Microscopy more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components 500 tests Live Cell Staining Buffer 1 x 50ml LysoGreen Indicator 1 x 100µl
- Research Areas
Our Abpromise guarantee covers the use of ab112136 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Fluorescence Microscopy||Fluorescence Microscopy|
CytoPainter Lysosomal Staining Kit - Green Fluorescence images
ab112136 staining U2OS cells by fluorescence microscopy (in a black 96 well plate).
References for CytoPainter Lysosomal Staining Kit - Green Fluorescence (ab112136)
ab112136 has not yet been referenced specifically in any publications.