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Product name
Cytochrome-C Oxidase (Complex IV) Human Profiling ELISA Kit
See all Complex IV products (10) ...
Specificity
Species– Human. Rat and Mouse samples are not appropriate, other species are untested.
Precision
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| Overall | 6 | 5.6% |
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| Overall | 6 | 10.9% |
Tests
1 x 96 test
Sample type
Tissue Extracts, Cell Lysate
Assay type
Sandwich
Sensitivity
20 µg/ml
Recovery
| Sample type | Average % | Range % |
|---|---|---|
| Tissue Extracts | 5 - 250 | |
| Cell Lysate | 20 - 1000 |
Cross reactivity
Reacts with
Human
Product overview
Cytochrome-c oxidase (Complex IV) is the forth enzyme of the oxidative phosphorylation (OXPHOS) system within the mitochondrial inner membrane. Cytochrome-c oxidase is a large protein complex of approximately 200,000 MW made up of 13 different subunits. Three catalytic subunits I,II, and III are encoded on mitochondrial DNA (mtDNA). All other subunits are encoded by nuclear genomic DNA, made in the cytosol, and translocated into the organelle for assembly at the inner membrane. Complex IV catalyses the pumping of protons across the inner membrane and the transfer of electrons to the terminal acceptor oxygen (O2) making water. Several cytochrome-c oxidase subunits have isoforms present in different tissues which subtly regulate enzymatic activity. Isoform expression or switching is likely in response to intracellular oxygen concentration.
ab124538 Cytochrome-c oxidase (Complex IV) Human Profiling kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the comparison of cytochrome-c oxidase levels or profile in cell and tissue lysates. The assay employs a human cytochrome-c oxidase specific capture antibody coated onto microplate well strips.
Samples are pipetted into the wells and cytochrome-c oxidase present in the sample is bound to the wells by the immobilized antibody. The wells are washed and an anti- cytochrome-c oxidase detector antibody is added. After washing away unbound detector antibody, an HRP-conjugated secondary antibody specific for the detector antibody is pipetted into the wells. The wells are again washed, an HRP substrate solution (TMB) is added to the wells and color develops in proportion to the amount of cytochrome-c oxidase bound. The developing blue color is measured at 600 nm.
Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.
Notes
Store all components at 4°C. This kit is stable for 6 months from receipt. Unused microplate strips should be returned to the pouch containing the desiccant and resealed.
Tested applications
Sandwich ELISAmore details
Properties
Storage instructions
Please see Notes section
| Components | Identifier | 1 x 96 tests |
|---|---|---|
| 10X Blocking Buffer | 8209802 | 1 x 6ml |
| 10X Cytochrome Oxidase Detector Antibody | 8209566 | 1 x 1ml |
| 10X HRP Label | 8206038 | 1 x 1ml |
| 20X Buffer | 8203013 | 1 x 20ml |
| Cytochrome Oxidase Microplate | 8209567 | 1 unit |
| Extraction Buffer | 8201093 | 1 x 15ml |
| TMB Development Solution | 8209803 | 1 x 12ml |
Research areas
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Cytochromes
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Kits/ Lysates/ Other >> Kits >> Cell Metabolism Kits >> Other Metabolism Assay
Signal Transduction >> Metabolism >> Mitochondrial
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Sandwich ELISA - Human Cytochrome Oxidase (Complex IV) Human Profiling ELISA Kit (ab124538)
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Sandwich ELISA - Human Cytochrome-C Oxidase (Complex IV) Human Profiling ELISA Kit (ab124538)
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Sandwich ELISA - Human Cytochrome-C Oxidase (Complex IV) Human Profiling ELISA Kit (ab124538)
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Alternative names
- Cytochrome c oxidase
- MS1038
Database links
- Entrez Gene: 4512 Human
- Entrez Gene: 4513 Human
- Entrez Gene: 4514 Human
- Entrez Gene: 1345 Human
- Entrez Gene: 1351 Human
- Entrez Gene: 1329 Human
- Entrez Gene: 1349 Human
- Entrez Gene: 1337 Human
see all
Applications
Show applications key
Our Abpromise guarantee covers the use of ab124538 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
sELISA
Cytochrome-C Oxidase (Complex IV) Human Profiling ELISA Kit images:
Sandwich ELISA - Human Cytochrome Oxidase (Complex IV) Human Profiling ELISA Kit (ab124538)
Example control sample curve of serially titrated HepG2 extract in the working range of the assay.
Sandwich ELISA - Human Cytochrome-C Oxidase (Complex IV) Human Profiling ELISA Kit (ab124538)
Human HepG2 cells were cultured in NARTI Zalcitabine (ddC) for 6 days to ensure a significant effect on mitochondrial DNA replication and mitochondrial protein translation. The drug reduced mitochondrial DNA levels and hence mitochondrial protein expression. As a consequence the assembly of Complexes I, III and IV were severely affected. Note that loss of the two small mitochondrial DNA encoded subunits of Complex V (ATP synthase) does not affect overall assembly. Interestingly an increase in Complex II was induced as a consequence of I, III, IV loss possibly to up regulate mitochondrial citric acid cycle function.
Sandwich ELISA - Human Cytochrome-C Oxidase (Complex IV) Human Profiling ELISA Kit (ab124538)
Human HepG2 cells were cultured in chloramphenicol for 6 days to ensure a significant effect on mitochondrial DNA replication and mitochondrial protein translation. The antibiotic inhibited mitochondrial protein translation and assembly of Complexes I and IV but had no significant effect on Complex II, III or V.
Protocols
References for Cytochrome-C Oxidase (Complex IV) Human Profiling ELISA Kit (ab124538)
ab124538 has not yet been referenced specifically in any publications.
Publishing research using ab124538? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"