![Western blot - Cytochrome C Oxidase subunit VIb antibody [3F9D3D11AF6 ] (ab110266) image](#Cytochrome-C-Oxidase-subunit-VIb-Primary-antibodies-ab110266-1.jpg)
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Product name
Anti-Cytochrome C Oxidase subunit VIb antibody [3F9D3D11AF6 ]
See all Cytochrome C Oxidase subunit VIb products (5) ...
Description
Mouse monoclonal [3F9D3D11AF6 ] to Cytochrome C Oxidase subunit VIb
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human
Immunogen
Bovine Complex IV (native holoenzyme protein, purified from heart)
Positive control
Human, cow, rat and mouse heart.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HBS
Concentration
Concentration information loading...
Purification notes
Near homogeneity as judged by SDS-PAGE. ab110266 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Clonality
Monoclonal
Clone number
3F9D3D11AF6
Isotype
IgG1
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Cytochromes
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Oxidative phosphorylation >> Complex IV
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Integration of energy
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Signal Transduction >> Metabolism >> Mitochondrial
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
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Western blot - Cytochrome C Oxidase subunit VIb antibody [3F9D3D11AF6 ] (ab110266)
![Western blot - Cytochrome C Oxidase subunit VIb antibody [3F9D3D11AF6 ] (ab110266) image](/ps/datasheet/images/110/ab110266/Cytochrome-C-Oxidase-subunit-VIb-Primary-antibodies-ab110266-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab110266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/ml. Predicted molecular weight: 10 kDa.
Target
Function
Connects the two COX monomers into the physiological dimeric form.
Involvement in disease
Defects in COX6B1 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
Sequence similarities
Belongs to the cytochrome c oxidase subunit 6B family.
Cellular localization
Mitochondrion intermembrane space.
Target information above from: UniProt accessionP14854
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- COX 6B antibody
- COX VIb 1 antibody
- COX VIb-1 antibody
see all
Database links
- Entrez Gene: 1340 Human
- Entrez Gene: 110323 Mouse
- Entrez Gene: 502592 Rat
- Omim: 124089 Human
- SwissProt: P14854 Human
- SwissProt: P56391 Mouse
- SwissProt: P80430 Rat
- Unigene: 431668 Human
see all
Anti-Cytochrome C Oxidase subunit VIb antibody [3F9D3D11AF6 ] images:
Western blot - Cytochrome C Oxidase subunit VIb antibody [3F9D3D11AF6 ] (ab110266)
All lanes : Anti-Cytochrome C Oxidase subunit VIb antibody [3F9D3D11AF6 ] (ab110266) at 1 µg/ml
Lane 1 : Isolated mitochondria from Human Heart at 5 µg
Lane 2 : Isolated mitochondria from Bovine Heart at 4 µg
Lane 3 : Isolated mitochondria from Rat Heart at 10 µg
Lane 4 : Isolated mitochondria from Mouse Heart at 10 µg
Predicted band size : 10 kDa
References for Anti-Cytochrome C Oxidase subunit VIb antibody [3F9D3D11AF6 ] (ab110266)
This product has been referenced in:
- Wong R et al. Cardioprotection leads to novel changes in the mitochondrial proteome. Am J Physiol Heart Circ Physiol 298:H75-91 (2010).Read more (PubMed: 19855063) »
- Yu Q et al. Differential loss of cytochrome-c oxidase subunits in ischemia-reperfusion injury: exacerbation of COI subunit loss by PKC-epsilon inhibition. Am J Physiol Heart Circ Physiol 294:H2637-45 (2008).Read more (PubMed: 18408135) »
See all 4 publications for this product
Publishing research using ab110266? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"