![Western blot - Cytochrome C antibody [7H8.2C12] (ab28137) image](#Cytochrome-C-Primary-antibodies-ab28137-1.jpg)
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Product name
Anti-Cytochrome C antibody [7H8.2C12]
See all Cytochrome C products (23) ...
Description
Mouse monoclonal [7H8.2C12] to Cytochrome C
Specificity
ab28137 recognises denatured cytochrome C. It does not recognize the native protein.
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Sheep, Rabbit, Horse, Guinea pig, Hamster, Cow, Human, Monkey
Immunogen
Full length protein: Horse Cytochrome C
Epitope
ab28137 reacts with an epitope located in the region within amino acids 93/104 of Cytochrome C
Positive control
HeLa cell lysate, Rat Brain Tissue Extract, Mouse Brain Tissue Extract.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
7H8.2C12
Isotype
IgG2b
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolism processes >> Apoptosis
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Cytochromes
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Integration of energy
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Energy Metabolism
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Lipid and lipoprotein metabolism >> Lipases
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Oxidative phosphorylation
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Cancer >> Invasion/microenvironment >> Apoptosis >> Cytochrome C
Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Metabolism >> Energy Metabolism
Cell Biology >> Apoptosis >> Mitochondrial
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cytochromes
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Western blot - Cytochrome C antibody [7H8.2C12] (ab28137)
![Western blot - Cytochrome C antibody [7H8.2C12] (ab28137) image](/ps/datasheet/images/28/ab28137/Cytochrome-C-Primary-antibodies-ab28137-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab28137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/500. This dilution was sufficient for detection of 20µg of HeLa heat shocked cell lysate, by colorimetric immunoblot analysis, using Goat Anti-Mouse IgG:AP as the secondary antibody. Predicted molecular weight: 12 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
Involvement in disease
Defects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation.
Sequence similarities
Belongs to the cytochrome c family.
Post-translational
modifications
Binds 1 heme group per subunit.
Cellular localization
Mitochondrion matrix.
Target information above from: UniProt accessionP99999
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CYC antibody
- CYC_HUMAN antibody
- CYCS antibody
see all
Database links
- Entrez Gene: 510767 Cow
- Entrez Gene: 54205 Human
- Entrez Gene: 100425223 Monkey
- Entrez Gene: 13063 Mouse
- Entrez Gene: 25309 Rat
- Omim: 123970 Human
- SwissProt: P62894 Cow
- SwissProt: P00004 Horse
see all
Anti-Cytochrome C antibody [7H8.2C12] images:
Western blot - Cytochrome C antibody [7H8.2C12] (ab28137)
All lanes : Anti-Cytochrome C antibody [7H8.2C12] (ab28137) at 1/200 dilution
Lane 1 : Primary murine myoblast lysate
Lane 2 : Primary murine myoblast lysate
Lane 3 : Primary murine myoblast lysate
Lane 4 : Primary murine myoblast lysate
Lysates/proteins at 50 µg per lane.
Secondary
HRP-conjugated goat anti-rabbit IgG at 1/10000 dilution
Predicted band size : 12 kDa
Primary myotubes were transduced with PPARδ shRNA or a control adenovirus. The next day, the cells were transduced with either the PGC-1α or GFP adenovirus for 48 hours. Cells were labeled with 14C-palmitate, and FAO rates were measured. 50µg of lysate was loaded per lane.
Image from Kleiner S et al, J Biol Chem. 2009 Jul 10;284(28):18624-33. Epub 2009 May 12, Fig 8. DOI 10.1074/jbc.M109.008797
References for Anti-Cytochrome C antibody [7H8.2C12] (ab28137)
This product has been referenced in:
- Wang H & Clem RJ The role of IAP antagonist proteins in the core apoptosis pathway of the mosquito disease vector Aedes aegypti. Apoptosis 16:235-48 (2011). WB.Read more (PubMed: 21274634) »
- Queiroga CS et al. Glutathionylation of adenine nucleotide translocase induced by carbon monoxide prevents mitochondrial membrane permeabilization and apoptosis. J Biol Chem 285:17077-88 (2010). WB; Rat.Read more (PubMed: 20348099) »
See all 5 publications for this product
Publishing research using ab28137? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"