Anti-Cytochrome C antibody (ab49879)

Overview

• Product nameAnti-Cytochrome C antibodySee all Cytochrome C primary antibodies ...
• Description
  Sheep polyclonal to Cytochrome C
• Tested applicationsWB, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Rat, Rabbit, Dog, Human
  
• Immunogen

  Full length native protein (purified) (Rabbit) conjugated to KLH

• Positive controlHuman heart tissue, whole extracts of MCF 7, Jurkat, Rat 1, and MDCK cells.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 15mM Sodium Azide
  Constituents: 1% BSA, 0.01M PBS, pH 7.4
• Concentration information loading...
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Oxidative phosphorylation

  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Cytochromes

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Integration of energy

  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Lipases

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Apoptosis

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Energy Metabolism

  • Cancer
  • Invasion/microenvironment
  • Apoptosis
  • Cytochrome C

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Integration of energy metabolism

  • Signal Transduction
  • Metabolism
  • Mitochondrial

  • Signal Transduction
  • Metabolism
  • Energy Metabolism

  • Cardiovascular
  • Lipids / Lipoproteins
  • Lipid Metabolism
  • Cytochromes

  • Cell Biology
  • Apoptosis
  • Mitochondrial

Applications

Target

• FunctionElectron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
  Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
• Involvement in diseaseDefects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation.
• Sequence similaritiesBelongs to the cytochrome c family.
• Post-translational
  modificationsBinds 1 heme group per subunit.
• Cellular localizationMitochondrion matrix.

Target information above from: UniProt accession P99999 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 475258 Dog
  • Entrez Gene: 54205 Human
  • Entrez Gene: 25309 Rat
  • Omim: 123970 Human
  • SwissProt: P00011 Dog
  • SwissProt: P99999 Human
  • SwissProt: P00008 Rabbit
  • SwissProt: P62898 Rat

  • Unigene: 437060 Human
  • Unigene: 617193 Human
  • Unigene: 2202 Rat

  see all

• Alternative names
  CYC antibodyCYC_HUMAN antibodyCYCS antibody

  Cytochrome c antibodyCytochrome c somatic antibodyHCS antibodyTHC4 antibody

  see all

Anti-Cytochrome C antibody images

• 

  Immunocytochemistry/ Immunofluorescence - Cytochrome C antibody (ab49879)
  ab49879 at 5 µg/ml staining Human MCF-7 cells. Cells were fixed and permeabilized with 3% paraformaldehyde followed by 0.5% TritonX-100 and visualised with Rabbit anti-Sheep IgG, FITC conjugate.