![Western blot - Cytochrome C oxidase subunit II antibody [ 4B12A5 ] (ab110271) image](#Cytochrome-C-oxidase-subunit-II-Primary-antibodies-ab110271-1.jpg)
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- Secondary antibodies
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Product name
Anti-Cytochrome C oxidase subunit II antibody [ 4B12A5 ]
See all Cytochrome C oxidase subunit II products (6) ...
Description
Mouse monoclonal [ 4B12A5 ] to Cytochrome C oxidase subunit II
Tested applications
Cross reactivity
Reacts with
Saccharomyces cerevisiae
Immunogen
Saccharomyces cerevisiae Cytochrome C oxidase subunit II
Positive control
Mitochondria from yeast membrane extract
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HBS
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Near homogeneity as judged by SDS-PAGE. ab110271 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Clonality
Monoclonal
Clone number
4B12A5
Isotype
IgG2a
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Cytochromes
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Oxidative phosphorylation >> Complex IV
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Integration of energy
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Integration of energy metabolism
Signal Transduction >> Metabolism >> Mitochondrial
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Western blot - Cytochrome C oxidase subunit II antibody [ 4B12A5 ] (ab110271)
![Western blot - Cytochrome C oxidase subunit II antibody [ 4B12A5 ] (ab110271) image](/ps/datasheet/images/110/ab110271/Cytochrome-C-oxidase-subunit-II-Primary-antibodies-ab110271-1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab110271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 2 µg/ml. Predicted molecular weight: 26 kDa.
Target
Function
Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1.
Involvement in disease
Defects in MT-CO2 are a cause of mitochondrial complex IV deficiency (MT-C4D) [MIM:220110]; also known as cytochrome c oxidase deficiency. A disorder of the mitochondrial respiratory chain with heterogeneous clinical manifestations, ranging from isolated myopathy to severe multisystem disease affecting several tissues and organs. Features include hypertrophic cardiomyopathy, hepatomegaly and liver dysfunction, hypotonia, muscle weakness, excercise intolerance, developmental delay, delayed motor development and mental retardation. A subset of patients manifest Leigh syndrome.
Sequence similarities
Belongs to the cytochrome c oxidase subunit 2 family.
Cellular localization
Mitochondrion inner membrane.
Target information above from: UniProt accessionP00403
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- COII antibody
- COX2 antibody
- COX2_HUMAN antibody
see all
Database links
- Entrez Gene: 285521 Saccharomyces cerevisiae
Anti-Cytochrome C oxidase subunit II antibody [ 4B12A5 ] images:
Western blot - Cytochrome C oxidase subunit II antibody [ 4B12A5 ] (ab110271)
Anti-Cytochrome C oxidase subunit II antibody [ 4B12A5 ] (ab110271) at 2 µg/ml + Mitochondria from yeast membrane extract at 10 µg
Predicted band size : 26 kDa
References for Anti-Cytochrome C oxidase subunit II antibody [ 4B12A5 ] (ab110271)
This product has been referenced in:
- Atkinson A et al. Mzm1 influences a labile pool of mitochondrial zinc important for respiratory function. J Biol Chem 285:19450-9 (2010).Read more (PubMed: 20404342) »
- Di Bella D et al. Mutations in the mitochondrial protease gene AFG3L2 cause dominant hereditary ataxia SCA28. Nat Genet 42:313-21 (2010).Read more (PubMed: 20208537) »
See all 26 publications for this product
Publishing research using ab110271? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"