Recombinant PE Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab223958)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR6293] to Cytochrome P450 17A1/CYP17A1
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
Overview
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Product name
PE Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293]
See all Cytochrome P450 17A1/CYP17A1 primary antibodies -
Description
PE Rabbit monoclonal [EPR6293] to Cytochrome P450 17A1/CYP17A1 -
Host species
Rabbit -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt (intra): HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6293 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab125022)
- Alexa Fluor® 647 Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab203743)
- Alexa Fluor® 488 Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab203995)
- HRP Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] (ab204022)
- Anti-Cytochrome P450 17A1/CYP17A1 antibody [EPR6293] - Low endotoxin, Azide free (ab226009)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223958 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/5000.
The cellular localisation of this product has been verified in ICC/IF. |
Notes |
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Flow Cyt (Intra)
1/5000. The cellular localisation of this product has been verified in ICC/IF. |
Target
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Function
Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty. -
Pathway
Lipid metabolism; steroid biosynthesis. -
Involvement in disease
Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5) [MIM:202110]. AH5 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: "salt wasting" (SW, the most severe type), "simple virilizing" (SV, less severely affected patients), with normal aldosterone biosynthesis, "non-classic form" or late onset (NC or LOAH), and "cryptic" (asymptomatic). -
Sequence similarities
Belongs to the cytochrome P450 family. -
Post-translational
modificationsPhosphorylation is necessary for 17,20-lyase, but not for 17-alpha-hydroxylase activity. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 1586 Human
- Omim: 609300 Human
- SwissProt: P05093 Human
- Unigene: 438016 Human
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Alternative names
- 20 lyase antibody
- CP17A_HUMAN antibody
- CPT7 antibody
see all
Images
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Overlay histogram showing HeLa cells stained with ab223958 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223958, 1/5000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab223958 has not yet been referenced specifically in any publications.