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Anti-Cytochrome P450 1A1 + 1A2 antibody (ab4227)

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1 question for ab4227

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Question 1

Thursday 22-April-2004

I am writing about a product I recently purchased from your company. I bought product code: ab4227, and I have a question about the suggest dilution factor. In the product insert, you mentioned that for WB a dilution of 1/100 is recommended. However, my detection system (luminol/chemiluminescence) requires a dilution of 1:1000 for my primary

antibody.

I tried it with a dilution of 1:1000 and it seems to work, what is the reason for specifying 1:100 dilution?

ANSWER:

  

Thank you for your enquiry.

If you load 0.2 µg of recombinant human cytochrome P450 (positive control), we would suggest using 1/100 dilution. The dilution depends on the type of the samples, the expressed levels of the target protein, detection system, the amount of protein loaded on to the gel etc. Usually the optimal dilution should be determined by the researcher.

We encourage customers to feed their results back to us, whether positive or negative, and make all information about each of our products available to researchers. In return we will forward a USD15/EUR15/GBP10 Amazon gift voucher.

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