Anti-CYP1A1 antibody (ab124295)
Key features and details
- Rabbit polyclonal to CYP1A1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CYP1A1 antibody
See all CYP1A1 primary antibodies -
Description
Rabbit polyclonal to CYP1A1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Dog, Pig, Chimpanzee, Macaque monkey, Chinese hamster, Orangutan -
Immunogen
Synthetic peptide within Human CYP1A1 aa 50-150 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available asab155750) -
Positive control
- This antibody gave a positive signal in both Human Lung and Placenta tissue lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124295 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 58 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 58 kDa). |
Target
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Function
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. -
Tissue specificity
Lung, lymphocytes and placenta. -
Sequence similarities
Belongs to the cytochrome P450 family. -
Cellular localization
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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Database links
- Entrez Gene: 1543 Human
- Entrez Gene: 403103 Pig
- Omim: 108330 Human
- SwissProt: P04798 Human
- Unigene: 72912 Human
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Alternative names
- AHH antibody
- AHRR antibody
- Aryl hydrocarbon hydroxylase antibody
see all
Images
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All lanes : Anti-CYP1A1 antibody (ab124295) at 1 µg/ml
Lane 1 : Lung (Human) Tissue Lysate
Lane 2 : Human placenta tissue lysate - total protein (ab29745)
Lane 3 : Lung (Human) Tissue Lysate with Immunising peptide at 1 µg/ml
Lane 4 : Human placenta tissue lysate - total protein (ab29745) with Immunising peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 73 kDa why is the actual band size different from the predicted?
Additional bands at: 48 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab124295 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab124295 has not yet been referenced specifically in any publications.