Cytochrome P450 2A6 293T Transfected Lysate - (positive control) (ab94284)

Overview

  • Product nameCytochrome P450 2A6 293T Transfected Lysate - (positive control)
  • General notesab94284 is a 293T cell transfected lysate in which Human Cytochrome P450 2A6 has been transiently over-expressed using a pCMV-Cytochrome P450 2A6 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsWBmore details

Properties

  • BackgroundFunction: Exhibits a high coumarin 7-hydroxylase activity. Can act in the hydroxylation of the anti-cancer drugs cyclophosphamide and ifosphamide. Competent in the metabolic activation of aflatoxin B1. Constitutes the major nicotine C-oxidase. Possesses low phenacetin O-deethylation activity. Tissue specificity: Liver. Similarity: Belongs to the cytochrome P450 family.

Applications

Our Abpromise guarantee covers the use of ab94284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Cytochrome P450 2A6 293T Transfected Lysate - (positive control) images

    • ab94284 at 15µg/lane on an SDS-PAGE gel.
    • All lanes : Anti-Cytochrome P450 2A6 antibody (ab89633) at 1/500 dilution

      Lane 1 : Cytochrome P450 2A6 293T Transfected Lysate - (positive control) (ab94284)
      Lane 2 : 293T Non Transfected Lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

    References for Cytochrome P450 2A6 293T Transfected Lysate - (positive control) (ab94284)

    ab94284 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"