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Cytochrome P450 2E1 293T Transfected Lysate - (positive control) (ab94254)

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Overview

  • Product nameCytochrome P450 2E1 293T Transfected Lysate - (positive control)
  • General notesab94254 is a 293T cell transfected lysate in which Human Cytochrome P450 2E1 has been transiently over-expressed using a pCMV-Cytochrome P450 2E1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • Tested applicationsWB more details

Properties

  • BackgroundFunction: Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. Similarity: Belongs to the cytochrome P450 family.

    • Applications

    Our Abpromise guarantee covers the use of ab94254 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB
  • Application notesWB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Cytochrome P450 2E1 293T Transfected Lysate - (positive control) images

    • SDS-PAGE - Cytochrome P450 2E1 293T Transfected Lysate - (positive control) (ab94254)
      SDS-PAGE - Cytochrome P450 2E1 293T Transfected Lysate - (positive control) (ab94254)
      ab94254 at 15µg/lane on an SDS-PAGE gel
    • Western blot - Cytochrome P450 2E1 293T Transfected Lysate - (positive control) (ab94254)
      Western blot - Cytochrome P450 2E1 293T Transfected Lysate - (positive control) (ab94254)
      All lanes : Anti-Cytochrome P450 2E1 antibody (ab90564) at 1/500 dilution

      Lane 1 : Cytochrome P450 2E1 293T Transfected Lysate - (positive control) (ab94254)
      Lane 2 : 293T non-transfected lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

    References for Cytochrome P450 2E1 293T Transfected Lysate - (positive control) (ab94254)

    ab94254 has not yet been referenced specifically in any publications.

    Publishing research using ab94254 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"